Synthesis of biotin-AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription (Q46260753)

20 December 2017

title

Synthesis of biotin-AMP conjugate for 5' biotin labeling of RNA through one-step in vitro transcription (English)

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20 December 2017

author name string

Faqing Huang

1

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20 December 2017

Jun He

2

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20 December 2017

Yilin Zhang

3

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20 December 2017

Yanlin Guo

4

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20 December 2017

publication date

1 January 2008

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20 December 2017

published in

Nature Protocols

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20 December 2017

volume

3

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20 December 2017

issue

12

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20 December 2017

page(s)

1848-1861

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20 December 2017

Ligand-induced conformational changes observed in single RNA molecules

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Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase

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Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates

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RNA-Catalyzed CoA, NAD, and FAD synthesis from phosphopantetheine, NMN, and FMN.

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Efficient incorporation of CoA, NAD and FAD into RNA by in vitro transcription

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Site-specific biotinylation of RNA molecules by transcription using unnatural base pairs

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T7 RNA polymerase produces 5' end heterogeneity during in vitro transcription from certain templates

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More mistakes by T7 RNA polymerase at the 5' ends of in vitro-transcribed RNAs

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Superior 5' homogeneity of RNA from ATP-initiated transcription under the T7 phi 2.5 promoter

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Novel cyanine-AMP conjugates for efficient 5' RNA fluorescent labeling by one-step transcription and replacement of [gamma-32P]ATP in RNA structural investigation

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Separation of complementary strands of plasmid DNA using the biotin-avidin system and its application to heteroduplex formation and RNA/DNA hybridizations in electron microscopy.

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Isolation of a ribozyme with 5'-5' ligase activity

21 January 2018

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RNA-catalyzed thioester synthesis

21 January 2018

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Ribozyme-catalysed amino-acid transfer reactions

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Non-isotopic RNA probes. Comparison between different labels and detection systems

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Interactions of HIV-1 TAR RNA with Tat-derived peptides discriminated by on-line acoustic wave detector

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Development of biosensors with aptamers as bio-recognition element: the case of HIV-1 Tat protein.

31 March 2020

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Optimization of aptamer microarray technology for multiple protein targets

31 March 2020

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Aptamers as molecular recognition elements in chromatographic separations.

31 March 2020

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Degree of biotinylation in nucleic acids estimated by a gel retardation assay

31 March 2020

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T7 RNA polymerase directed expression of the Escherichia coli rrnB operon

31 March 2020

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Nonradioactive 3'-end-labeling of RNA molecules of different lengths by terminal deoxynucleotidyltransferase

31 March 2020

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Construction of folate-conjugated pRNA of bacteriophage phi29 DNA packaging motor for delivery of chimeric siRNA to nasopharyngeal carcinoma cells

31 March 2020

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31 March 2020

Identifiers

DOI

10.1038/NPROT.2008.185

1 reference

20 December 2017

1 reference

20 December 2017