Homologous expression and purification of mutants of an essential protein by reverse epitope-tagging (Q47941086)

6 February 2018

title

Homologous expression and purification of mutants of an essential protein by reverse epitope-tagging (English)

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6 February 2018

author name string

Thomann HU

1

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6 February 2018

Ibba M

2

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6 February 2018

Hong KW

3

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6 February 2018

Söll D

4

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6 February 2018

publication date

1 January 1996

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6 February 2018

published in

Bio/technology

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6 February 2018

volume

14

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6 February 2018

issue

1

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6 February 2018

page(s)

50-55

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6 February 2018

A C-terminal signal prevents secretion of luminal ER proteins

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RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus

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Synthesis and sequence-specific proteolysis of a hybrid protein (colicin A::growth hormone releasing factor) produced in Escherichia coli

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The structure of an antigenic determinant in a protein

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Nucleotide sequences of the Escherichia coli nagE and nagB genes: the structural genes for the N-acetylglucosamine transport protein of the bacterial phosphoenolpyruvate: sugar phosphotransferase system and for glucosamine-6-phosphate deaminase

21 January 2018

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Structural basis of anticodon loop recognition by glutaminyl-tRNA synthetase

21 January 2018

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Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs

21 January 2018

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Purification of His-Tag fusion proteins from Escherichia coli

21 January 2018

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Expression of heterologous proteins in Escherichia coli

21 January 2018

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7 January 2021

Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations.

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7 January 2021

Switching recognition of two tRNA synthetases with an amino acid swap in a designed peptide

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7 January 2021

Design of a membrane protein for site-specific proteolysis: properties of engineered factor Xa protease sites in the lactose permease of Escherichia coli

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7 January 2021

Site-directed mutagenesis to fine-tune enzyme specificity

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7 January 2021

Efficient introduction of cloned mutant alleles into the Escherichia coli chromosome

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7 January 2021

Organisation of the Escherichia coli chromosome between genes glnS and glnU, V

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7 January 2021

Sequence of the nagBACD operon in Escherichia coli K12 and pattern of transcription within the nag regulon

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Characterization of a urea induced molten globule intermediate state of glutaminyl-tRNA synthetase from Escherichia coli.

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7 January 2021

Cloning, expression in Escherichia coli, and reconstitution of human myoglobin

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7 January 2021

Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN'

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7 January 2021

PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli

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7 January 2021

A Short Polypeptide Marker Sequence Useful for Recombinant Protein Identification and Purification

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7 January 2021

Generation of protein-reactive antibodies by short peptides is an event of high frequency: implications for the structural basis of immune recognition.

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7 January 2021

Glutaminyl-tRNA synthetase of Escherichia coli

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7 January 2021

Rapid and efficient site-specific mutagenesis without phenotypic selection

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7 January 2021

Identifiers

DOI

10.1038/NBT0196-50

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6 February 2018

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6 February 2018