Digitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA. (Q50508130)

14 March 2018

title

Digitonin-permeabilization of astrocytes in culture monitored by trypan blue exclusion and loss of S100B by ELISA. (English)

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14 March 2018

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Tramontina F

1

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Karl J

2

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Gottfried C

3

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Mendez A

4

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Gonçalves D

5

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Portela LV

6

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Gonçalves CA

7

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14 March 2018

publication date

1 November 2000

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14 March 2018

published in

Brain Research Protocols

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volume

6

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issue

1-2

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page(s)

86-90

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Regulation of protein phosphorylation in astrocyte cultures by external calcium ions: specific effects on the phosphorylation of glial fibrillary acidic protein (GFAP), vimentin and heat shock protein 27 (HSP27).

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Loss and Ca(2+)-dependent retention of scinderin in digitonin-permeabilized chromaffin cells: correlation with Ca(2+)-evoked catecholamine release

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Regulated exocytotic secretion from permeabilized cells

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Astrocytes possess prostaglandin F2α receptors coupled to phospholipase C

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Cell membrane resealing by a vesicular mechanism similar to neurotransmitter release

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Impaired Ca-signaling in astrocytes from the Ts16 mouse model of Down syndrome.

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Calcium-modulated proteins change their immunoreactivity in the presence of Ca2+: a study of antibody recognition in a dot immunoassay for calmodulin, calcineurin (beta-subunit), and S100B.

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Simultaneous measurement of tyrosine hydroxylase activity and phosphorylation in bovine adrenal chromaffin cells

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Identifiers

DOI

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14 March 2018

PubMed ID

11086267

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14 March 2018