Comparison of Metallothionein Detection by Using Brdicka Reaction and Enzyme-Linked Immunosorbent Assay Employing Chicken Yolk Antibodies (Q62399625)

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Pavlina Blahova

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Johana Nakielna

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Ivo Fabrik

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Vojtech Adam

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Tomas Eckschlager

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Miroslava Beklova

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Zdenka Svobodova

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Vratislav Horak

Metallothioneins with unusual residues: histidines as modulators of zinc affinity and reactivity

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publication date

December 2009

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published in

Electroanalysis

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volume

21

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issue

23

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page(s)

2575-2583

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cites work

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Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues.

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Biochemistry of metallothionein

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Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin

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69 Overview of metallothionein

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Metallothionein in physiological and physiopathological processes

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Metallothionein: the multipurpose protein

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Augmented expression of metallothionein and glutathione S-transferase pi as unfavourable prognostic factors in cisplatin-treated ovarian cancer patients.

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Metallothionein in bladder cancer: correlation of overexpression with poor outcome after chemotherapy

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Nuclear metallothionein expression correlates with cisplatin resistance of ovarian cancer cells and poor clinical outcome.

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Employment of Electrochemical Techniques for Metallothionein Determination in Tumor Cell Lines and Patients with a Tumor Disease

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Metallothionein expression is increased in monocytes and erythrocytes of young men during zinc supplementation

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Metallothionein - overexpression as a highly significant prognostic factor in melanoma: a prospective study on 1270 patients

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Effects of Reduced Glutathione, Surface Active Agents, and Ionic Strength on the Detection of Metallothioneins by Using of Brdicka Reaction

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Recent developments in quantification methods for metallothionein

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Advances in analytical methodology for bioinorganic speciation analysis: metallomics, metalloproteomics and heteroatom-tagged proteomics and metabolomics

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Analysis for metallothioneins using coupled techniques

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Hyphenated techniques for the characterization and quantification of metallothionein isoforms

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9 Determination of metallothionein in biological materials

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Quantification of oxidized metallothionein in biological material by a Cd saturation method

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Quantitation of Cu-containing metallothionein by a Cd-saturation method

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Quantification of cadmium-induced metallothionein in crustaceans by the silver-saturation method

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12 Quantification of metallothionein by silver saturation

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A modified mercury saturation assay for measuring metallothionein

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A sensitive time-resolved fluorescent immunoassay for metallothionein protein

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Quantification of metallothionein isoforms using an enzyme-linked immunosorbent assay (ELISA) with two specific antisera

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Measurement of cadmium-induced metallothionein in urine by ELISA and prevention of overestimation due to polymerization

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Separation and quantitation of metallothionein isoforms from liver of untreated rats by ion-exchange high-performance liquid chromatography and atomic absorption spectrometry

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Metallothionein induction by Cu, Cd and Hg in Dicentrarchus labrax liver: assessment by RP-HPLC with fluorescence detection and spectrophotometry.

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Multidimensional approaches in biochemical speciation analysis.

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Strategies for the qualitative and quantitative analysis of metallothionein isoforms by capillary electrophoresis

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Characterization and quantification of metallothionein isoforms by capillary electrophoresis-inductively coupled plasma-isotope-dilution mass spectrometry.

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Comparison of different techniques for the analysis of metallothionein isoforms by capillary electrophoresis

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Species analysis of metallothionein isoforms in human brain cytosols by use of capillary electrophoresis hyphenated to inductively coupled plasma-sector field mass spectrometry.

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Determination of sulfur and selected trace elements in metallothionein-like proteins using capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry with an octopole reaction cell

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Utilizing a chronopotentiometric sensor technique for metallothionein determination in fish tissues and their host parasites

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Differential pulse anodic stripping voltammetry detection of metallothionein at bismuth film electrodes

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Evaluation of different purification procedures for the electrochemical quantification of mussel metallothioneins

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The induction of metallothionein in tissues of the Norway lobster Nephrops norvegicus following exposure to cadmium, copper and zinc: the relationships between metallothionein and the metals

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Electrochemical study of heavy metals and metallothionein in yeast Yarrowia lipolytica.

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Electrochemical detection of metallothionein

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Quantitative reverse transcription polymerase chain reaction of a molluscan metallothionein mRNA

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Localization and quantification of Cd- and Cu-specific metallothionein isoform mRNA in cells and organs of the terrestrial gastropod Helix pomatia

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Effects of cadmium on metallothionein-I and metallothionein-II mRNA expression in rat ventral, lateral, and dorsal prostatic lobes: quantification by competitive RT-PCR

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Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: monitoring gene expression using cDNA microarrays combined with real-time RT-PCR.

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Metallothioneins and cancer

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Evidence for a high molecular weight cytosolic factor that binds brain and liver metallothionein.

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Antiserum specific for the intact isoform-3 of metallothionein

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Enzyme-immunoassay for the determination of metallothionein in human urine: application to environmental monitoring

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Heterogeneity of antibodies to metallothionein isomers and development of a simple enzyme‐linked immunosorbent assay

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Monoclonal antibodies against metallothioneins from the human liver

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Development of ELISA for metallothionein-II allows determination of heavy metal pollution of fresh water.

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Metallothionein mRNA in monocytes and peripheral blood mononuclear cells and in cells from dried blood spots increases after zinc supplementation of men

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Antibodies to proteins from yolk of immunized hens

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Efficient production of chicken egg yolk antibodies against a conserved mammalian protein

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Influence of Cadmium(II) Ions and Brewery Sludge on Metallothionein Level in Earthworms (Eisenia fetida) - Bio- transforming of Toxic Wastes

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Metallothionein levels in sperm of various fish species

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Attomole voltammetric determination of metallothionein

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An Electrochemical Detection of Metallothioneins at the Zeptomole Level in Nanolitre Volumes.

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Evaluation of differential pulse polarography for the quantification of metallothionein--a comparison with RIA

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Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels

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Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion

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High-Performance Liquid Chromatography with Coulometric Electrode Array Detector for the Determination of Quercetin Levels in Cells of the Immune System

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16 Detection of metallothionein by western blotting

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Brdicka-type processes of cysteine and cysteine-containing peptides on silver amalgam electrodes.

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Determination of Cu-containing metallothionein: comparison of Ag saturation assay, thiomolybdate assay, and enzyme-linked immunosorbent assay

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Comparison of methods of metallothionein quantification: cadmium radioassay, mercury radioassay, and radioimmunoassay.

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