Development and optimization of a single-step procedure using protein A affinity chromatography to isolate murine IgG1 monoclonal antibodies from hybridoma supernatants (Q67842164)

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scientific article published on November 29, 1991

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English	Development and optimization of a single-step procedure using protein A affinity chromatography to isolate murine IgG1 monoclonal antibodies from hybridoma supernatants	scientific article published on November 29, 1991

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scholarly article

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

Development and optimization of a single-step procedure using protein A affinity chromatography to isolate murine IgG1 monoclonal antibodies from hybridoma supernatants (English)

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

monoclonal antibody

1 reference

based on heuristic

inferred from title

organic chemistry

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

analytical chemistry

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Gerhard Schuler

1

object named as

G. Schuler

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1783662

22 November 2022

author name string

M. Reinacher

2

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?resulttype=core&format=json&query=SRC%3AMED%20AND%20EXT_ID%3A1783662

22 November 2022

language of work or name

1 reference

National Center for Biotechnology Information

PubMed publication ID

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=1783662

22 November 2022

publication date

1 November 1991

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

29 November 1991

1 reference

National Center for Biotechnology Information

PubMed publication ID

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=1783662

22 November 2022

full work available at URL

https://api.elsevier.com/content/article/PII:002196739185198O?httpAccept=text/xml

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

https://api.elsevier.com/content/article/PII:002196739185198O?httpAccept=text/plain

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Journal of Chromatography

587

1

61–70

publication date

29 November 1991

1 reference

National Center for Biotechnology Information

PubMed publication ID

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db=pubmed&retmode=json&id=1783662

22 November 2022

587

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

1

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

61-70

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

One-step purification of monoclonal IgG antibodies from mouse ascites. An evaluation of different adsorption techniques using high performance liquid chromatography

1

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

A method suitable for the isolation of monoclonal antibodies from large volumes of serum-containing hybridoma cell culture supernatants

2

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Monoclonal antibody production in semi-continuous serum- and protein-free culture. Effect of glutamine concentration and culture conditions on cell growth and antibody secretion

4

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Kinetics of monoclonal antibody production in low serum growth medium

5

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

In vitro production of monoclonal antibodies under serum-free conditions using a compact and inexpensive hollow fibre cell culture unit

6

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Optimisation of hybridoma cell growth and monoclonal antibody secretion in a chemically defined, serum- and protein-free culture medium

12

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Purification of in vitro produced mouse monoclonal antibodies. A two-step procedure utilizing cation exchange chromatography and gel filtration

13

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Routine large-scale production of monoclonal antibodies in a protein-free culture medium

14

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Serum-free medium for growth factor-dependent and -independent plasmacytomas and hybridomas

15

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Use of protein A to remove immunoglobulins from serum in hybridoma culture media

16

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Biomedica biochimica acta

19

42

278

author name string

Eigenbrodt

publication date

1983

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Prediction of the performance of preparative affinity chromatography

20

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-Sepharose

21

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

High-performance liquid affinity chromatographic separation of mouse monoclonal antibodies with protein A silica

23

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Temperature affects binding of murine monoclonal IgG antibodies to protein A

26

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Mouse IgG1 heterogeneity: variable binding of monoclonal IgG1 antibodies to protein A-sepharose

27

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Protein A Reactivity of Various Mammalian Immunoglobulins

28

1 reference

10.1016/0021-9673(91)85198-O

https://api.crossref.org/works/10.1016/0021-9673(91)85198-O

22 November 2022

Identifiers

10.1016/0021-9673(91)85198-O

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:1783662%20AND%20SRC:MED&resulttype=core&format=json

18 September 2019

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