Cloning, expression and purification of a recombinant poly-histidine-linked HIV-1 protease (Q72815656)

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29 October 2019

title

Cloning, expression and purification of a recombinant poly-histidine-linked HIV-1 protease (English)

1 reference

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29 October 2019

author name string

A Leuthardt

series ordinal

1

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29 October 2019

J L Roesel

series ordinal

2

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29 October 2019

publication date

1 July 1993

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29 October 2019

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29 October 2019

volume

326

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29 October 2019

issue

1-3

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29 October 2019

page(s)

275-280

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Active human immunodeficiency virus protease is required for viral infectivity

29 October 2019

cites work

1 reference

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Expression and processing of the AIDS virus reverse transcriptase in Escherichia coli

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An 11-kDa form of human immunodeficiency virus protease expressed in Escherichia coli is sufficient for enzymatic activity

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High-level expression of self-processed HIV-1 protease in Escherichia coli using a synthetic gene

21 January 2018

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Substitution mutations of the highly conserved arginine 87 of HIV-1 protease result in loss of proteolytic activity

21 January 2018

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High-level synthesis of recombinant HIV-1 protease and the recovery of active enzyme from inclusion bodies

21 January 2018

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Chemical synthesis and enzymatic activity of a 99-residue peptide with a sequence proposed for the human immunodeficiency virus protease

21 January 2018

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Enzymatic activity of a synthetic 99 residue protein corresponding to the putative HIV-1 protease

21 January 2018

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A T5 promoter-based transcription-translation system for the analysis of proteins in vitro and in vivo.

21 January 2018

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Genetic Approach to Facilitate Purification of Recombinant Proteins with a Novel Metal Chelate Adsorbent

21 January 2018

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DNA sequencing with chain-terminating inhibitors

21 January 2018

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A fluorometric assay for HIV-protease activity using high-performance liquid chromatography

21 January 2018

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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa

21 January 2018

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New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues

21 January 2018

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Autoprocessing of the HIV-1 protease using purified wild-type and mutated fusion proteins expressed at high levels in Escherichia coli

21 January 2018

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Human immunodeficiency viral protease is catalytically active as a fusion protein: characterization of the fusion and native enzymes produced in Escherichia coli

21 January 2018

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Substrate analogue inhibition and active site titration of purified recombinant HIV-1 protease

21 January 2018

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10.1016/0014-5793(93)81807-C

21 January 2018

Identifiers

DOI

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29 October 2019

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