Development of a simple and rapid assay for the evaluation of inhibitors of human 17alpha-hydroxylase-C(17,20)-lyase (P450cl7) by coexpression of P450cl7 with NADPH-cytochrome-P450-reductase in Escherichia coli (Q73443514)

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scientific article published on 01 December 2000

Language	Label	Description	Also known as
English	Development of a simple and rapid assay for the evaluation of inhibitors of human 17alpha-hydroxylase-C(17,20)-lyase (P450cl7) by coexpression of P450cl7 with NADPH-cytochrome-P450-reductase in Escherichia coli	scientific article published on 01 December 2000

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scholarly article

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

Development of a simple and rapid assay for the evaluation of inhibitors of human 17alpha-hydroxylase-C(17,20)-lyase (P450cl7) by coexpression of P450cl7 with NADPH-cytochrome-P450-reductase in Escherichia coli (English)

1 reference

Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

Escherichia coli

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author name string

P B Ehmer

1

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

J Jose

2

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

R W Hartmann

3

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4 November 2019

publication date

1 December 2000

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4 November 2019

The Journal of Steroid Biochemistry and Molecular Biology

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

75

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

1

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

57-63

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

Microsomal cytochrome P-450 from neonatal pig testis: two enzymatic activities (17 alpha-hydroxylase and c17,20-lyase) associated with one protein

1 reference

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Cytochrome P-450 C21scc: one enzyme with two actions: hydroxylase and lyase

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20-amino and 20,21-aziridinyl pregnene steroids: development of potent inhibitors of 17 alpha-hydroxylase/C17,20-lyase (P450 17)

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Synthesis and evaluation of novel steroidal oxime inhibitors of P450 17 (17 alpha-hydroxylase/C17-20-lyase) and 5 alpha-reductase types 1 and 2.

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Synthesis of novel oximes of 2-aryl-6-methoxy-3,4-dihydronaphthalene and their evaluation as inhibitors of 17 alpha-hydroxylase-C17,20-lyase (P450 17)

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Pyridyl substituted benzocycloalkenes: new inhibitors of 17 alpha-hydroxylase/17,20-lyase (P450 17 alpha)

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Imidazole substituted biphenyls: a new class of highly potent and in vivo active inhibitors of P450 17 as potential therapeutics for treatment of prostate cancer

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Novel imidazolyl and triazolyl substituted biphenyl compounds: synthesis and evaluation as nonsteroidal inhibitors of human 17alpha-hydroxylase-C17, 20-lyase (P450 17)

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Expression and enzymatic activity of recombinant cytochrome P450 17 alpha-hydroxylase in Escherichia coli

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Coexpression of mammalian cytochrome P450 and reductase in Escherichia coli

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The function of recombinant cytochrome P450s in intact Escherichia coli cells: the 17 alpha-hydroxylation of progesterone and pregnenolone by P450c17.

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High efficiency transformation of Escherichia coli with plasmids

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An improved automated biuret method for the determination of microgram protein concentrations

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Cytochrome P450c17-expressing Escherichia coli as a first-step screening system for 17alpha-hydroxylase-C17,20-lyase inhibitors

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Identifiers

10.1016/S0960-0760(00)00137-0

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:11179909%20AND%20SRC:MED&resulttype=core&format=json

4 November 2019

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