Functional recognition of 5' splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly (Q74307271)

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10 November 2019

title

Functional recognition of 5' splice site by U4/U6.U5 tri-snRNP defines a novel ATP-dependent step in early spliceosome assembly (English)

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10 November 2019

author name string

P A Maroney

series ordinal

1

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10 November 2019

C M Romfo

series ordinal

2

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10 November 2019

T W Nilsen

series ordinal

3

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10 November 2019

publication date

1 August 2000

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10 November 2019

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10 November 2019

volume

6

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10 November 2019

page(s)

317-328

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10 November 2019

issue

2

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Cross-intron bridging interactions in the yeast commitment complex are conserved in mammals

10 November 2019

cites work

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A U1/U4/U5 snRNP complex induced by a 2'-O-methyl-oligoribonucleotide complementary to U5 snRNA.

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Pre-mRNA splicing in vitro requires intact U4/U6 small nuclear ribonucleoprotein

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U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6

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The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein

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Insertion of part of an intron into the 5' untranslated region of a Caenorhabditis elegans gene converts it into a trans-spliced gene

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Mutations in the conserved loop of human U5 snRNA generate use of novel cryptic 5' splice sites in vivo

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A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1-independent splicing

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The 5' splice site consensus RNA oligonucleotide induces assembly of U2/U4/U5/U6 small nuclear ribonucleoprotein complexes

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Mechanisms for selecting 5' splice sites in mammalian pre-mRNA splicing

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U1 snRNA promotes the selection of nearby 5' splice sites by U6 snRNA in mammalian cells

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U4/U5/U6 snRNP recognizes the 5' splice site in the absence of U2 snRNP.

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Disruption of base pairing between the 5' splice site and the 5' end of U1 snRNA is required for spliceosome assembly

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Splicing factor Prp8 governs U4/U6 RNA unwinding during activation of the spliceosome

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Rapid and efficient site-specific mutagenesis without phenotypic selection

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Antisense probes containing 2-aminoadenosine allow efficient depletion of U5 snRNP from HeLa splicing extracts

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A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding

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The human Prp8 protein is a component of both U2- and U12-dependent spliceosomes

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An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway

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The U1 small nuclear RNA-protein complex selectively binds a 5' splice site in vitro

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Mutations in yeast U5 snRNA alter the specificity of 5' splice-site cleavage

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RNA-RNA interactions in the spliceosome: unraveling the ties that bind

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The invariant U5 snRNA loop 1 sequence is dispensable for the first catalytic step of pre-mRNA splicing in yeast

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7 January 2021

RNA unwinding in U4/U6 snRNPs requires ATP hydrolysis and the DEIH-box splicing factor Brr2

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Specific and stable intron-factor interactions are established early during in vitro pre-mRNA splicing.

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Conserved loop I of U5 small nuclear RNA is dispensable for both catalytic steps of pre-mRNA splicing in HeLa nuclear extracts.

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An RNA switch at the 5' splice site requires ATP and the DEAD box protein Prp28p

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Functional reconstitution of U6 snRNA in nematode cis- and trans-splicing: U6 can serve as both a branch acceptor and a 5' exon

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The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing

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Identifiers

DOI

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10 November 2019

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