In vivo biotinylated recombinant antibodies: high efficiency of labelling and application to the cloning of active anti-human IgG1 Fab fragments (Q77838795)

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scientific article published on 01 April 1999

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English	In vivo biotinylated recombinant antibodies: high efficiency of labelling and application to the cloning of active anti-human IgG1 Fab fragments	scientific article published on 01 April 1999

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scholarly article

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10357213%20AND%20SRC:MED&resulttype=core&format=json

8 December 2019

In vivo biotinylated recombinant antibodies: high efficiency of labelling and application to the cloning of active anti-human IgG1 Fab fragments (English)

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Europe PubMed Central

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10357213%20AND%20SRC:MED&resulttype=core&format=json

8 December 2019

author name string

A P Sibler

1

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8 December 2019

E Kempf

2

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8 December 2019

A Glacet

3

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8 December 2019

G Orfanoudakis

4

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8 December 2019

D Bourel

5

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8 December 2019

E Weiss

6

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8 December 2019

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1 April 1999

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8 December 2019

Journal of Immunological Methods

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8 December 2019

224

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8 December 2019

1-2

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8 December 2019

129-140

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8 December 2019

Bacterial secretion of the Fab fragment of a mouse monoclonal IgM that reacts with IgG variable regions

1 reference

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7 January 2021

Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing

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7 January 2021

Genetic and biochemical characterization of the birA gene and its product: evidence for a direct role of biotin holoenzyme synthetase in repression of the biotin operon in Escherichia coli

1 reference

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Comparative interaction kinetics of two recombinant Fabs and of the corresponding antibodies directed to the coat protein of tobacco mosaic virus

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7 January 2021

Affinity chromatographic purification of antibodies to a biotinylated fusion protein expressed in Escherichia coli

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Antigen detection using recombinant, bifunctional single-chain Fv fusion proteins synthesised in Escherichia coli.

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7 January 2021

Nucleotide sequence of the birA gene encoding the biotin operon repressor and biotin holoenzyme synthetase functions of Escherichia coli

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7 January 2021

Recombinant antibody fragments

1 reference

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7 January 2021

Single-chain Fv fusion proteins suitable as coating and detecting reagents in a double antibody sandwich enzyme-linked immunosorbent assay

1 reference

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7 January 2021

Recombinant single-chain Fv fragments carrying C-terminal cysteine residues: Production of bivalent and biotinylated miniantibodies

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7 January 2021

Simple affinity purification of antibodies using in vivo biotinylation of a fusion protein

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7 January 2021

Bacterially expressed Fabs of monoclonal antibodies neutralizing tumour necrosis factor alpha in vitro retain full binding and biological activity

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7 January 2021

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The nucleotide sequence of pACYC177.

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Lupus-derived autoantibodies with dual autoactivity: anti-DNA and anti-Fc. II. Fine specificity of anti-self autoreactivity

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A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli

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A versatile plasmid expression vector for the production of biotinylated proteins by site-specific, enzymatic modification in Escherichia coli

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7 January 2021

Vectors for a 'double-tagging' assay for protein-protein interactions: localization of the CDK2-binding domain of human p21.

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7 January 2021

Specific immobilization of firefly luciferase through a biotin carboxyl carrier protein domain

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7 January 2021

Discovery of human antibodies to cell surface antigens by capture lift screening of phage-expressed antibody libraries

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7 January 2021

In vivo biotinylated recombinant antibodies: construction, characterization, and application of a bifunctional Fab-BCCP fusion protein produced in Escherichia coli.

1 reference

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7 January 2021

Application of an alkaline phosphatase fusion protein system suitable for efficient screening and production of Fab-enzyme conjugates in Escherichia coli.

1 reference

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7 January 2021

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7 January 2021

Identifiers

10.1016/S0022-1759(99)00016-2

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Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10357213%20AND%20SRC:MED&resulttype=core&format=json

8 December 2019

PubMed publication ID

1 reference

Europe PubMed Central

PubMed publication ID

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:10357213%20AND%20SRC:MED&resulttype=core&format=json

8 December 2019

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