Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants (Q25257169)

6 July 2017

title

Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants (English)

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6 July 2017

James C Samuelson

1

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6 July 2017

Richard D Morgan

2

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6 July 2017

Jack S Benner

3

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6 July 2017

Toby E Claus

4

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6 July 2017

Stephanie L Packard

5

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6 July 2017

Shuang-yong Xu

6

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6 July 2017

language of work or name

English

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publication date

2 February 2006

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6 July 2017

published in

Nucleic Acids Research

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6 July 2017

volume

34

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6 July 2017

issue

3

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6 July 2017

page(s)

796-805

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In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination

6 July 2017

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7 April 2017

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7 April 2017

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7 April 2017

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7 April 2017

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7 April 2017

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7 April 2017

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7 April 2017

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7 April 2017

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Specificity changes in the evolution of type II restriction endonucleases: a biochemical and bioinformatic analysis of restriction enzymes that recognize unrelated sequences

7 April 2017

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The isolation of strand-specific nicking endonucleases from a randomized SapI expression library

28 September 2017

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Assessing the plasticity of DNA target site recognition of the PI-SceI homing endonuclease using a bacterial two-hybrid selection system

28 September 2017

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28 September 2017

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28 September 2017

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28 September 2017

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28 September 2017

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Functional analysis of putative restriction-modification system genes in the Helicobacter pylori J99 genome

28 September 2017

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Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities

28 September 2017

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The 'endo-blue method' for direct cloning of restriction endonuclease genes in E. coli.

28 September 2017

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Saturation mutagenesis of His114 of EcoRI reveals relaxed-specificity mutants

28 September 2017

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Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli

28 September 2017

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28 September 2017

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Mutants of the EcoRI endonuclease with promiscuous substrate specificity implicate residues involved in substrate recognition

28 September 2017

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Engineering of restriction endonucleases: using methylation activity of the bifunctional endonuclease Eco57I to select the mutant with a novel sequence specificity

28 September 2017

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Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity

28 November 2018

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Mutational analysis of the function of Met137 and Ile197, two amino acids implicated in sequence-specific DNA recognition by the EcoRI endonuclease.

28 November 2018

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Catalytic and DNA binding properties of PvuII restriction endonuclease mutants

28 November 2018

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12 December 2020

Protein engineering of the restriction endonuclease EcoRV . Structure-guided design of enzyme variants that recognize the base pairs flanking the recognition site

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12 December 2020

Genetic analysis of the base-specific contacts of BamHI restriction endonuclease

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12 December 2020

Implications for switching restriction enzyme specificities from the structure of BstYI bound to a BglII DNA sequence

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12 December 2020

On the possibilities and limitations of rational protein design to expand the specificity of restriction enzymes: a case study employing EcoRV as the target

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12 December 2020

YidC mediates membrane protein insertion in bacteria

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The characterization and cloning of the EagI restriction-modification system

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SOS induction as an in vivo assay of enzyme-DNA interactions

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Identifiers

DOI

10.1093/NAR/GKJ483

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6 July 2017

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6 July 2017

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