Purified Argonaute2 and an siRNA form recombinant human RISC (Q28242666)

2

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Gregory Hannon

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object named as

Gregory J Hannon

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author name string

Fabiola V Rivas

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Ji-Joon Song

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Juan P Aragon

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Jidong Liu

Nature Structural & Molecular Biology

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language of work or name

English

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publication date

April 2005

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published in

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volume

12

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issue

4

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page(s)

340-9

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Single-stranded antisense siRNAs guide target RNA cleavage in RNAi

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Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs

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Argonaute2 is the catalytic engine of mammalian RNAi

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Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain

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The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes

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Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate

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RNA interference is mediated by 21- and 22-nucleotide RNAs

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Crystal structure of a phospholipase D family member

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Structural biochemistry of a type 2 RNase H: RNA primer recognition and removal during DNA replication

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Two-metal ion mechanism of RNA cleavage by HIV RNase H and mechanism-based design of selective HIV RNase H inhibitors

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Three-dimensional structure of the Tn5 synaptic complex transposition intermediate

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Evidence for "unseen" transposase--DNA contacts

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Two-metal active site binding of a Tn5 transposase synaptic complex

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Binding of different divalent cations to the active site of avian sarcoma virus integrase and their effects on enzymatic activity

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Mechanisms of gene silencing by double-stranded RNA

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Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination

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An extensively modified version of MolScript that includes greatly enhanced coloring capabilities

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Raster3D Version 2.0. A program for photorealistic molecular graphics

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An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

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ATP requirements and small interfering RNA structure in the RNA interference pathway

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Crystal structure of Argonaute and its implications for RISC slicer activity

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Bi-cycling the furin pathway: from TGN localization to pathogen activation and embryogenesis

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Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways

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Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity

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RISC is a 5' phosphomonoester-producing RNA endonuclease

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The RNA-induced silencing complex is a Mg2+-dependent endonuclease.

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13 Laser cross-linking of protein-nucleic acid complexes

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Nucleic acid 3'-end recognition by the Argonaute2 PAZ domain

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A protein sensor for siRNA asymmetry

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Crystal structure of the ribonuclease H domain of HIV-1 reverse transcriptase

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Catalysis by Escherichia coli ribonuclease HI is facilitated by a phosphate group of the substrate.

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A general two-metal-ion mechanism for catalytic RNA

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Crystal structure of Escherichia coli RNase HI in complex with Mg2+ at 2.8 A resolution: proof for a single Mg(2+)-binding site

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Structure/function insights into Tn5 transposition.

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Tn5 transposase active site mutations suggest position of donor backbone DNA in synaptic complex

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Kinetic analysis of the RNAi enzyme complex

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A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins

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The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans

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RISC assembly defects in the Drosophila RNAi mutant armitage.

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Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs.

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In vitro analysis of RNA interference in Drosophila melanogaster.

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Processing of X-ray diffraction data collected in oscillation mode

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Molecular basis of double-stranded RNA-protein interactions: structure of a dsRNA-binding domain complexed with dsRNA.

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Thermal stability of Escherichia coli ribonuclease HI and its active site mutants in the presence and absence of the Mg2+ ion. Proposal of a novel catalytic role for Glu48

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Structural basis for the 3'-5' exonuclease activity of Escherichia coli DNA polymerase I: a two metal ion mechanism

31 March 2020

1 reference

RNA helicase MUT-14-dependent gene silencing triggered in C. elegans by short antisense RNAs

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Electron-density map interpretation

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