Protein structure modeling indicates hexahistidine-tag interference with enzyme activity (Q30319684)

17 June 2017

Protein structure modeling indicates hexahistidine-tag interference with enzyme activity (English)

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17 June 2017

Anna-Carolin Freydank

series ordinal

1

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17 June 2017

Wolfgang Brandt

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2

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17 June 2017

Birgit Dräger

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3

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17 June 2017

language of work or name

English

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publication date

1 July 2008

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17 June 2017

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17 June 2017

72

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17 June 2017

1

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17 June 2017

173-183

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Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

17 June 2017

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https://api.crossref.org/works/10.1002%2FPROT.21905

A critical review of the methods for cleavage of fusion proteins with thrombin and factor Xa.

21 January 2018

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https://api.crossref.org/works/10.1002%2FPROT.21905

His-tag impact on structure.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Attachment of histidine tags to recombinant tumor necrosis factor-alpha drastically changes its properties.

21 January 2018

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https://api.crossref.org/works/10.1002%2FPROT.21905

Hexa-histidin tag position influences disulfide structure but not binding behavior of in vitro folded N-terminal domain of rat corticotropin-releasing factor receptor type 2a

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Identification of conserved lipid/detergent-binding sites in a high-resolution structure of the membrane protein cytochrome c oxidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Acetyltransfer in natural product biosynthesis--functional cloning and molecular analysis of vinorine synthase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Purification and partial amino acid sequences of the enzyme vinorine synthase involved in a crucial step of ajmaline biosynthesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Functional analysis of norcoclaurine synthase in Coptis japonica.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

(R,S)-Reticuline 7-O-methyltransferase and (R,S)-norcoclaurine 6-O-methyltransferase of Papaver somniferum - cDNA cloning and characterization of methyl transfer enzymes of alkaloid biosynthesis in opium poppy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Molecular cloning and characterization of norcoclaurine synthase, an enzyme catalyzing the first committed step in benzylisoquinoline alkaloid biosynthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

A polyketide synthase of Plumbago indica that catalyzes the formation of hexaketide pyrones

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Analgesia: morphine-pathway block in top1 poppies.

21 January 2018

1 reference

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New genes in alkaloid metabolism and transport

21 January 2018

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Plant cell factories in the post-genomic era: new ways to produce designer secondary metabolites

21 January 2018

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Chemistry and biology of calystegines

21 January 2018

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Short-chain dehydrogenases/reductases (SDR): the 2002 update

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Two tropinone reductases with different stereospecificities are short-chain dehydrogenases evolved from a common ancestor

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Two tropinone reducing enzymes from Datura stramonium transformed root cultures

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Molecular cloning, expression and characterization of tropinone reductase II, an enzyme of the SDR family in Solanum tuberosum (L.).

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Nutrient requirements of suspension cultures of soybean root cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Overexpression of tropinone reductases alters alkaloid composition in Atropa belladonna root cultures

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Calystegines in wild and cultivated Erythroxylum species.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Amino acid substitution matrices from protein blocks

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

All-atom empirical potential for molecular modeling and dynamics studies of proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Calculation of conformational ensembles from potentials of mean force. An approach to the knowledge-based prediction of local structures in globular proteins.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Calystegines in Calystegia sepium derive from the tropane alkaloid pathway

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Biosynthesis of calystegines: 15N NMR and kinetics of formation in root cultures of Calystegia sepium

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Capturing enzyme structure prior to reaction initiation: tropinone reductase-II-substrate complexes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Crystal structures of two tropinone reductases: different reaction stereospecificities in the same protein fold

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Analysis of bacterial glucose dehydrogenase homologs from thermoacidophilic archaeon Thermoplasma acidophilum: finding and characterization of aldohexose dehydrogenase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Structural insights into unique substrate selectivity of Thermoplasma acidophilum D-aldohexose dehydrogenase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FPROT.21905

Properties of short-chain dehydrogenase/reductase RalR1: characterization of purified enzyme, its orientation in the microsomal membrane, and distribution in human tissues and cell lines.

21 January 2018

1 reference

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Human recombinant prolidase from eukaryotic and prokaryotic sources. Expression, purification, characterization and long-term stability studies

21 January 2018

1 reference

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21 January 2018

Identifiers

DOI

10.1002/PROT.21905

1 reference

17 June 2017

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