Why the need for qPCR publication guidelines?--The case for MIQE. (Q34089254)

30 July 2017

Why the need for qPCR publication guidelines?--The case for MIQE. (English)

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30 July 2017

Stephen A Bustin

1

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30 July 2017

16 December 2009

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30 July 2017

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30 July 2017

50

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30 July 2017

217-226

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30 July 2017

4

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Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

30 July 2017

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.

7 January 2021

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Quantitative real-time RT-PCR--a perspective.

7 January 2021

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Reliability of real-time reverse-transcription PCR in clinical diagnostics: gold standard or substandard?

7 January 2021

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Molecular medicine, gene-expression profiling and molecular diagnostics: putting the cart before the horse

7 January 2021

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The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments

7 January 2021

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Studies on polynucleotides. XCVI. Repair replications of short synthetic DNA's as catalyzed by DNA polymerases

7 January 2021

1 reference

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Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

7 January 2021

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Model based analysis of real-time PCR data from DNA binding dye protocols.

7 January 2021

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Eleven golden rules of quantitative RT-PCR.

7 January 2021

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Real-time quantitative RT-PCR: design, calculations, and statistics.

7 January 2021

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Towards a systematic validation of references in real-time rt-PCR

7 January 2021

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Transcriptome profiling using next-generation sequencing

7 January 2021

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7 January 2021

Direct RNA sequencing

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Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts

7 January 2021

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Editorial: characterization and analysis of heterogeneity in biological systems

7 January 2021

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Noise in gene expression: origins, consequences, and control

7 January 2021

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Living with noisy genes: how cells function reliably with inherent variability in gene expression

7 January 2021

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Stochastic mRNA synthesis in mammalian cells

7 January 2021

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Quantification of multiple gene expression in individual cells

7 January 2021

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Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels

7 January 2021

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Stochastic protein expression in individual cells at the single molecule level

7 January 2021

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Stochasticity in gene expression: from theories to phenotypes

7 January 2021

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Increased cell-to-cell variation in gene expression in ageing mouse heart

7 January 2021

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Intracellular expression profiles measured by real-time PCR tomography in the Xenopus laevis oocyte

7 January 2021

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Measures of degeneracy and redundancy in biological networks

7 January 2021

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Quantification of mRNA in single cells and modelling of RT-qPCR induced noise

7 January 2021

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Stochastic modelling for quantitative description of heterogeneous biological systems.

7 January 2021

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The dynamic landscapes of RNA architecture

7 January 2021

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The spliceosome: design principles of a dynamic RNP machine

7 January 2021

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Pre-mRNA processing reaches back to transcription and ahead to translation

7 January 2021

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Small RNAs as guardians of the genome

7 January 2021

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Origins and Mechanisms of miRNAs and siRNAs

7 January 2021

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Phosphorylation of the human microRNA-generating complex mediates MAPK/Erk signaling

7 January 2021

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Evolution and functions of long noncoding RNAs

7 January 2021

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The antisense transcriptomes of human cells

7 January 2021

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mRNA localization: gene expression in the spatial dimension

7 January 2021

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15000 unique zebrafish EST clusters and their future use in microarray for profiling gene expression patterns during embryogenesis

7 January 2021

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Allele-specific expression patterns reveal biases and embryo-specific parent-of-origin effects in hybrid maize.

7 January 2021

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Allele-specific up-regulation of FGFR2 increases susceptibility to breast cancer

7 January 2021

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Allelic imbalance in BRCA1 and BRCA2 gene expression is associated with an increased breast cancer risk.

7 January 2021

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Design of studies using DNA microarrays

7 January 2021

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Quantification using real-time PCR technology: applications and limitations

7 January 2021

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RTPrimerDB: the portal for real-time PCR primers and probes

7 January 2021

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RTPrimerDB: the real-time PCR primer and probe database, major update 2006.

7 January 2021

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

RTPrimerDB: the real-time PCR primer and probe database

7 January 2021

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Standardized determination of real-time PCR efficiency from a single reaction set-up

7 January 2021

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A new mathematical model for relative quantification in real-time RT-PCR

7 January 2021

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Comparison of reverse transcriptases in gene expression analysis.

7 January 2021

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Properties of the reverse transcription reaction in mRNA quantification

7 January 2021

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SPUD: a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations.

7 January 2021

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Comparison of relative mRNA quantification models and the impact of RNA integrity in quantitative real-time RT-PCR.

7 January 2021

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RNA integrity and the effect on the real-time qRT-PCR performance

7 January 2021

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Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants.

7 January 2021

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A novel and universal method for microRNA RT-qPCR data normalization

7 January 2021

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Real-time RT-PCR normalisation; strategies and considerations.

7 January 2021

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The obesity susceptibility gene Cpe links FoxO1 signaling in hypothalamic pro-opiomelanocortin neurons with regulation of food intake

7 January 2021

1 reference

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Unreliable real-time PCR analysis of human endogenous retrovirus-W (HERV-W) RNA expression and DNA copy number in multiple sclerosis.

7 January 2021

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Molecular staging of colorectal cancer: new paradigm or waste of time?

7 January 2021

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Modern biomedical research: an internally self-consistent universe with little contact with medical reality?

7 January 2021

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Interlaboratory comparison of epstein-barr virus viral load assays

7 January 2021

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Interlaboratory comparison of cytomegalovirus viral load assays.

7 January 2021

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Multicenter comparison of different real-time PCR assays for quantitative detection of Epstein-Barr virus

7 January 2021

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Multicenter Trials Need To Use the Same Assay for Hepatitis C Virus Viral Load Determination

7 January 2021

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis

7 January 2021

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Real-time reverse transcription PCR and the detection of occult disease in colorectal cancer

7 January 2021

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Nucleic acid quantification and disease outcome prediction in colorectal cancer

7 January 2021

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Combined use of positive and negative immunomagnetic isolation followed by real-time RT-PCR for detection of the circulating tumor cells in patients with colorectal cancers.

7 January 2021

1 reference

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Quantitative detection of CEA expressing free tumor cells in the peripheral blood of colorectal cancer patients during surgery with real-time RT-PCR on a LightCycler

7 January 2021

1 reference

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Differential expression of carcinoembryonic antigen (CEA) splice variants in whole blood of colon cancer patients and healthy volunteers: implication for the detection of circulating colon cancer cells

7 January 2021

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MGMT promoter methylation and field defect in sporadic colorectal cancer.

7 January 2021

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Limitations of specific reverse-transcriptase polymerase chain reaction markers in the detection of metastases in the lymph nodes and blood of breast cancer patients.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Quantitative relationship of the circulating tumor burden assessed by reverse transcription-polymerase chain reaction for cytokeratin 19 mRNA in peripheral blood of colorectal cancer patients with Dukes' stage, serum carcinoembryonic antigen level a

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Reliability of quantitative reverse-transcriptase-PCR-based detection of tumour cells in the blood between different laboratories using a standardised protocol

7 January 2021

1 reference

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Quantitative real-time RT-PCR for detection of disseminated tumor cells in peripheral blood of patients with colorectal cancer using different mRNA markers.

7 January 2021

1 reference

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The mRNA of the Arabidopsis gene FT moves from leaf to shoot apex and induces flowering

7 January 2021

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7 January 2021

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Potential viral pathogenic mechanism for new variant inflammatory bowel disease

7 January 2021

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Absence of detectable measles virus genome sequence in blood of autistic children who have had their MMR vaccination during the routine childhood immunization schedule of UK.

7 January 2021

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No evidence of persisting measles virus in the intestinal tissues of patients with inflammatory bowel disease

7 January 2021

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No evidence of persisting measles virus in peripheral blood mononuclear cells from children with autism spectrum disorder

7 January 2021

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Lack of association between measles virus vaccine and autism with enteropathy: a case-control study

7 January 2021

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Why most published research findings are false

7 January 2021

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Early extreme contradictory estimates may appear in published research: the Proteus phenomenon in molecular genetics research and randomized trials

7 January 2021

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Most published research findings are false-but a little replication goes a long way

7 January 2021

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Minimizing mistakes and embracing uncertainty

7 January 2021

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When should potentially false research findings be considered acceptable?

7 January 2021

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Methodology capture: discriminating between the "best" and the rest of community practice

7 January 2021

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Design and optimization of reverse-transcription quantitative PCR experiments

7 January 2021

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Impact of RNA quality on reference gene expression stability.

7 January 2021

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Correcting false gene expression measurements from degraded RNA using RTQ-PCR.

7 January 2021

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Quantification of mRNA using real-time RT-PCR

7 January 2021

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

7 January 2021

1 reference

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Analyzing real-time PCR data by the comparative C(T) method

7 January 2021

1 reference

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Statistical methods for efficiency adjusted real-time PCR quantification.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Statistical analysis of real-time PCR data

7 January 2021

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https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Standardisation of data from real-time quantitative PCR methods - evaluation of outliers and comparison of calibration curves

7 January 2021

1 reference

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Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications

7 January 2021

1 reference

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Mathematics of quantitative kinetic PCR and the application of standard curves

7 January 2021

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A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR.

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

qPCR-DAMS: a database tool to analyze, manage, and store both relative and absolute quantitative real-time PCR data

7 January 2021

1 reference

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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

qpcR: an R package for sigmoidal model selection in quantitative real-time polymerase chain reaction analysis

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FJ.YMETH.2009.12.006

Statistical significance of quantitative PCR

7 January 2021

1 reference

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Towards sound epistemological foundations of statistical methods for high-dimensional biology

7 January 2021

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10.1016/J.YMETH.2009.12.006

7 January 2021

Identifiers

DOI

1 reference

30 July 2017

1 reference

PubMed ID

20025972