Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution (Q34365202)

1 August 2017

1 reference

title

Engineering proteins for thermostability: the use of sequence alignments versus rational design and directed evolution (English)

1 reference

1 August 2017

0 references

0 references

0 references

1 reference

Lehmann M

1

1 reference

PubMed ID

11551465

1 August 2017

Wyss M

series ordinal

2

1 reference

PubMed ID

11551465

1 August 2017

language of work or name

1 reference

1 August 2001

1 reference

Current Opinion in Biotechnology

1 August 2017

1 reference

1 August 2017

12

1 reference

1 August 2017

371-375

1 reference

1 August 2017

4

1 reference

Protein engineering of subtilisin

1 August 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Step-wise mutation of barnase to binase. A procedure for engineering increased stability of proteins and an experimental analysis of the evolution of protein stability

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Intrabody construction and expression. I. The critical role of VL domain stability

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Protein engineering of subtilisins to improve stability in detergent formulations

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

High resistance of Escherichia coli ribonuclease HI variant with quintuple thermostabilizing mutations to thermal denaturation, acid denaturation, and proteolytic degradation.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Design and structural analysis of an engineered thermostable chicken lysozyme

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Two exposed amino acid residues confer thermostability on a cold shock protein

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Molecular determinants of xylose isomerase thermal stability and activity: analysis of thermozymes by site-directed mutagenesis

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Factors enhancing protein thermostability

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Temperature adaptation of enzymes: lessons from laboratory evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Rational Modification of Protein Stability by the Mutation of Charged Surface Residues†

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Simultaneous enhancement of thermostability and catalytic activity of phospholipase A(1) by evolutionary molecular engineering

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Directed evolution of a thermostable esterase.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Directed evolution of thermostable kanamycin-resistance gene: a convenient selection marker for Thermus thermophilus

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Novel family shuffling methods for the in vitro evolution of enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

A structural view of evolutionary divergence

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Directed evolution converts subtilisin E into a functional equivalent of thermitase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Tryptophan phosphorescence study of enzyme flexibility and unfolding in laboratory-evolved thermostable esterases

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Directed evolution study of temperature adaptation in a psychrophilic enzyme

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Combinatorial and computational challenges for biocatalyst design

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Engineering an enzyme to resist boiling

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Design, structure and stability of a hyperthermophilic protein variant

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

DNA shuffling method for generating highly recombined genes and evolved enzymes

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Sequence statistics reliably predict stabilizing mutations in a protein domain

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Beta-turn propensities as paradigms for the analysis of structural motifs to engineer protein stability

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Intrabody construction and expression. II. A synthetic catalytic Fv fragment

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Intrabody construction and expression III: engineering hyperstable V(H) domains

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Semirational design of active tumor suppressor p53 DNA binding domain with enhanced stability

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Design of highly stable functional GroEL minichaperones.

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Stabilization of GroEL minichaperones by core and surface mutations

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

Directed evolution of a fungal peroxidase

1 reference

https://api.crossref.org/works/10.1016%2FS0958-1669%2800%2900229-9

7 January 2021

10.1016/S0958-1669(00)00229-9

Identifiers

DOI

1 reference

1 August 2017

1 reference

PubMed ID

11551465