Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila (Q34605914)

3 August 2017

Enhanced specificity and efficiency of the CRISPR/Cas9 system with optimized sgRNA parameters in Drosophila (English)

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3 August 2017

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Xingjie Ren

1

1 reference

3 August 2017

Zhihao Yang

2

1 reference

3 August 2017

Jiang Xu

3

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3 August 2017

Jin Sun

4

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3 August 2017

Decai Mao

5

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3 August 2017

Yanhui Hu

6

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3 August 2017

Su-Juan Yang

7

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3 August 2017

Huan-Huan Qiao

8

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3 August 2017

Xia Wang

9

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3 August 2017

Qun Hu

10

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3 August 2017

Patricia Deng

11

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3 August 2017

Lu-Ping Liu

12

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3 August 2017

Jun-Yuan Ji

13

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3 August 2017

Jin Billy Li

14

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3 August 2017

Jian-Quan Ni

15

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3 August 2017

23 October 2014

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3 August 2017

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3 August 2017

9

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3 August 2017

1151-1162

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3 August 2017

3

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Performance of the Cas9 nickase system in Drosophila melanogaster

3 August 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Various applications of TALEN- and CRISPR/Cas9-mediated homologous recombination to modify the Drosophila genome

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Efficient gene knock-out and knock-in with transgenic Cas9 in Drosophila

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Structures of Cas9 Endonucleases Reveal RNA-Mediated Conformational Activation

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Highly specific and efficient CRISPR/Cas9-catalyzed homology-directed repair in Drosophila

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Genetic screens in human cells using the CRISPR-Cas9 system

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Optimized gene editing technology for Drosophila melanogaster using germ line-specific Cas9.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Accelerated homologous recombination and subsequent genome modification in Drosophila.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Highly improved gene targeting by germline-specific Cas9 expression in Drosophila

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

DNA targeting specificity of RNA-guided Cas9 nucleases

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Highly efficient genome modifications mediated by CRISPR/Cas9 in Drosophila.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Highly efficient targeted mutagenesis of Drosophila with the CRISPR/Cas9 system.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Heritable genome editing in C. elegans via a CRISPR-Cas9 system

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 nuclease.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

RNA-guided human genome engineering via Cas9

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Multiplex genome engineering using CRISPR/Cas systems

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

A powerful method combining homologous recombination and site-specific recombination for targeted mutagenesis in Drosophila

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Ends-out, or replacement, gene targeting in Drosophila

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Identification of a nonhistone chromosomal protein associated with heterochromatin in Drosophila melanogaster and its gene

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

The heterochromatin-associated protein HP-1 is an essential protein in Drosophila with dosage-dependent effects on position-effect variegation

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Efficient and specific modifications of the Drosophila genome by means of an easy TALEN strategy.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Highly sensitive, fluorescent transformation marker for Drosophila transgenesis.

26 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4250831

Gene targeting by homologous recombination in Drosophila

26 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25437567

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1016/J.CELREP.2014.09.044

1 reference

3 August 2017

1 reference

3 August 2017

1 reference