Advances in real-time PCR: application to clinical laboratory diagnostics (Q36342551)

13 August 2017

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title

Advances in real-time PCR: application to clinical laboratory diagnostics (English)

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13 August 2017

Bernhard Kaltenboeck

1

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13 August 2017

Chengming Wang

series ordinal

2

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13 August 2017

publication date

1 January 2005

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Advances in Clinical Chemistry

13 August 2017

published in

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13 August 2017

volume

40

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13 August 2017

page(s)

219-259

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The LightCycler: a microvolume multisample fluorimeter with rapid temperature control.

13 August 2017

cites work

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12 December 2020

Identification and characterization of immunoglobulin G in blood as a major inhibitor of diagnostic PCR.

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12 December 2020

High temperature cDNA synthesis by AMV reverse transcriptase improves the specificity of PCR.

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12 December 2020

Rapid genotypic detection of Bacillus anthracis and the Bacillus cereus group by multiplex real-time PCR melting curve analysis

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12 December 2020

Rapid detection of VHL exon deletions using real-time quantitative PCR.

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12 December 2020

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

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12 December 2020

Detection of vaccinia virus, herpes simplex virus, varicella-zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving: implications for biosafety of bioterrorism agents

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12 December 2020

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12 December 2020

Boosting the sensitivity of real-time polymerase-chain-reaction testing for SARS.

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12 December 2020

High-resolution genotyping by amplicon melting analysis using LCGreen

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12 December 2020

Ultrarapid prenatal detection of Down syndrome using real-time multiplex polymerase chain reaction (PCR) in amniotic fluid

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12 December 2020

Sensitive method for detection of human herpesviruses 6 and 7 in saliva collected in field studies

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12 December 2020

Rapid screening of leukemia fusion transcripts in acute leukemia by real-time PCR.

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12 December 2020

Multiplex PCR detection of clinical and environmental strains of Vibrio vulnificus in shellfish.

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12 December 2020

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

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12 December 2020

Light-up probes: thiazole orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution.

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12 December 2020

Risk free simultaneous prenatal identification of fetal Rhesus D status and sex by multiplex real-time PCR using cell free fetal DNA in maternal plasma

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12 December 2020

Molecular beacons: probes that fluoresce upon hybridization

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12 December 2020

Rapid pathotyping of Newcastle disease virus (NDV) using fluorogenic probes in a PCR assay

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12 December 2020

Quantification of virulence-associated gene transcripts in epidemic methicillin resistant Staphylococcus aureus by real-time PCR.

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12 December 2020

Development of a combined selection and enrichment PCR procedure for Clostridium botulinum Types B, E, and F and its use to determine prevalence in fecal samples from slaughtered pigs

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12 December 2020

Biological sample collection and processing for molecular epidemiological studies

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12 December 2020

Mycoplasma pneumoniae and Legionella pneumophila in community-acquired lower respiratory tract infections among hospitalized children: diagnosis by real time PCR.

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12 December 2020

Direct comparison of GAPDH, beta-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia

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12 December 2020

The candidate oncogene ZNF217 is frequently amplified in colon cancer.

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12 December 2020

Genotyping of the MTHFR gene polymorphism, C677T in patients with leukemia by melting curve analysis

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12 December 2020

Specific detection of Stachybotrys chartarum in pure culture using quantitative polymerase chain reaction

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12 December 2020

Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions

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12 December 2020

Detection of human cytomegalovirus DNA by real-time quantitative PCR.

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12 December 2020

Rapid and sensitive routine detection of all members of the genus enterovirus in different clinical specimens by real-time PCR

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12 December 2020

Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction

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12 December 2020

Rapid combined genotyping assay for four achondroplasia and hypochondroplasia mutations by real-time PCR with multiple detection probes

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12 December 2020

Fluorescence spectral overlap compensation for any number of flow cytometry parameters

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12 December 2020

Rapid and sensitive identification of pathogenic and apathogenic Bacillus anthracis by real-time PCR.

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12 December 2020

Capacity of nine thermostable DNA polymerases To mediate DNA amplification in the presence of PCR-inhibiting samples

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12 December 2020

Quantitative and rapid detection of the trichloroethylene-degrading bacterium Methylocystis sp. M in groundwater by real-time PCR.

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12 December 2020

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase

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12 December 2020

Real-time PCR coupled with automated DNA extraction and detection of galactomannan antigen in serum by enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis

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12 December 2020

Real-time quantitative PCR-based serum neutralization test for detection and titration of neutralizing antibodies to chicken anemia virus

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12 December 2020

High-resolution semi-quantitative real-time PCR without the use of a standard curve

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12 December 2020

Development of a primary tamarin hepatocyte culture system for GB virus-B: a surrogate model for hepatitis C virus

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12 December 2020

Continuous fluorescence monitoring of rapid cycle DNA amplification

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12 December 2020

Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification

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12 December 2020

Multicolor molecular beacons for allele discrimination

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12 December 2020

Robotic automation performs a nested RT-PCR analysis for HCV without introducing sample contamination

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12 December 2020

Multiplex real-time PCR for detection of anaplasma phagocytophilum and Borrelia burgdorferi

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12 December 2020

Detection of PCR products using self-probing amplicons and fluorescence

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12 December 2020

Effectiveness and limitations of uracil-DNA glycosylases in sensitive real-time PCR assays.

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12 December 2020

Development of a real-time quantitative RT-PCR for the detection of HIV-2 RNA in plasma

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12 December 2020

Identification of DRB alleles in rhesus monkeys using polymerase chain reaction-sequence-specific primers (PCR-SSP) amplification

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12 December 2020

LightCycler-based quantitative PCR for rapid detection of human herpesvirus 6 DNA in clinical material

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12 December 2020

Spectral genotyping of human alleles

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12 December 2020

Effect of inhibitors in clinical specimens on Taq and Tth DNA polymerase-based PCR amplification of influenza A virus

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12 December 2020

Cell-free fetal DNA and intact fetal cells in maternal blood circulation: implications for first and second trimester non-invasive prenatal diagnosis

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12 December 2020

A real time PCR assay for the detection and quantitation of Mycobacterium avium subsp. paratuberculosis using SYBR Green and the Light Cycler

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12 December 2020

Rapid and specific detection of the Mycobacterium tuberculosis complex using fluorogenic probes andreal-time PCR

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12 December 2020

Assessment of methods for tissue-based detection of the HER-2/neu alteration in human breast cancer: a direct comparison of fluorescence in situ hybridization and immunohistochemistry

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12 December 2020

Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay

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12 December 2020

Optimization of the PCR for detection of Staphylococcus aureus nuc gene in bovine milk

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12 December 2020

Toward rapid real-time molecular diagnostic to guide smart use of antimicrobials

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12 December 2020

Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.

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12 December 2020

Development of fluorescent adjacent hybridization probes and their application in real-time PCR for the simultaneous detection and identification of Fervidobacterium and Caloramator

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12 December 2020

Evaluation of MDM2 and CDK4 amplification by real-time PCR on paraffin wax-embedded material: a potential tool for the diagnosis of atypical lipomatous tumours/well-differentiated liposarcomas

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12 December 2020

Mathematics of quantitative kinetic PCR and the application of standard curves

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12 December 2020

A new minor groove binding asymmetric cyanine reporter dye for real-time PCR.

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12 December 2020

Quantitation of varicella-zoster virus DNA in patients with Ramsay Hunt syndrome and zoster sine herpete

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12 December 2020

Development of a real-time PCR procedure including an internal control for the measurement of HCMV viral load

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12 December 2020

Inhibition and facilitation of nucleic acid amplification

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12 December 2020

Quantification of hepatitis C virus in human liver and serum samples by using LightCycler reverse transcriptase PCR.

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12 December 2020

Utility of random amplified polymorphic DNA PCR and TaqMan automated detection in molecular identification of Aspergillus fumigatus

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12 December 2020

Rapid and specific detection of PCR products using light-up probes

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12 December 2020

Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications

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12 December 2020

Use of fluorescence resonance energy transfer hybridization probes to evaluate quantitative real-time PCR for diagnosis of ocular toxoplasmosis

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12 December 2020

Highly sensitive and specific fluorescence reverse transcription-PCR assay for the pseudogene-free detection of beta-actin transcripts as quantitative reference

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12 December 2020

An homogeneous fluorescence polymerase chain reaction assay to identify Salmonella.

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12 December 2020

p73 is over-expressed in vulval cancer principally as the Delta 2 isoform

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12 December 2020

Typing and subtyping of 83 clinical isolates purified from surgically implanted silicone feeding tubes by random amplified polymorphic DNA amplification

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12 December 2020

Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia

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12 December 2020

Measurement of SIL-TAL1 fusion gene transcripts associated with human T-cell lymphocytic leukemia by real-time reverse transcriptase-PCR

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12 December 2020

Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis

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12 December 2020

MHC-linked olfactory receptor loci exhibit polymorphism and contribute to extended HLA/OR-haplotypes

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12 December 2020

Quantitative detection of Chlamydia spp. by fluorescent PCR in the LightCycler

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12 December 2020

The unusual origin of the polymerase chain reaction

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12 December 2020

Touch-down method for high-performance sequencing of polymerase chain reaction products

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12 December 2020

Detection and genotyping of oocysts of Cryptosporidium parvum by real-time PCR and melting curve analysis

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12 December 2020

Detection of Cryptosporidium parvum DNA in formed human feces by a sensitive PCR-based assay including uracil-N-glycosylase inactivation

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12 December 2020

Rapid prenatal diagnosis of aneuploidy by quantitative fluorescent PCR on fetal samples from mothers at high risk for chromosome disorders

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12 December 2020

Application of a real-time polymerase chain reaction with internal positive control for detection and quantification of enterovirus in cerebrospinal fluid

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12 December 2020

Amplification and protein expression of chromosome 12q13-15 genes in osteosarcomas of the jaws

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12 December 2020

p53 and human cancer: the first ten thousand mutations.

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12 December 2020

Trypanosomatid biodiversity in Costa Rica: genotyping of parasites from Heteroptera using the spliced leader RNA gene

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12 December 2020

Genotyping of feline MHC (FLA) class II DRB by PCR-RFLP method using group-specific primers.

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12 December 2020

A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics

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12 December 2020

Real-time PCR method for detection of Encephalitozoon intestinalis from stool specimens

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12 December 2020

Lack of association between TTV viral load and aminotransferase levels in patients with hepatitis C or non-B-C

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12 December 2020

Improved indicators for assessing the reliability of detection and quantification by kinetic PCR

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12 December 2020

Accurate and rapid prenatal diagnosis of the most frequent East Mediterranean beta-thalassemia mutations.

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12 December 2020

Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications

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12 December 2020

Development of a real time quantitative PCR assay for detection of porcine endogenous retrovirus

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12 December 2020

Development of a real-time quantitative assay for detection of Epstein-Barr virus.

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12 December 2020

TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase.

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12 December 2020

A new mathematical model for relative quantification in real-time RT-PCR

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12 December 2020

Increased fetal DNA concentrations in the plasma of pregnant women carrying fetuses with trisomy 21

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12 December 2020

Real-time PCR in the microbiology laboratory

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12 December 2020

One-step real-time duplex reverse transcription PCRs simultaneously quantify analyte and housekeeping gene mRNAs

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12 December 2020

Rapid real time PCR to distinguish between high risk human papillomavirus types 16 and 18.

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12 December 2020

Complex polysaccharides as PCR inhibitors in feces: Helicobacter pylori model

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12 December 2020

Reverse transcriptase. The use of cloned Moloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA.

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12 December 2020

Amplification and overexpression of the IGF2 regulator PLAG1 in hepatoblastoma

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12 December 2020

Mitochondrial DNA, human evolution and the cancer genotype

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12 December 2020

Efficient production of single-stranded DNA as long as 2 kb for sequencing of PCR-amplified DNA

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12 December 2020

Temperature non-homogeneity in rapid airflow-based cycler significantly affects real-time PCR

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12 December 2020

Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes.

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12 December 2020

Identification of widespread Helicobacter hepaticus infection in feces in commercial mouse colonies by culture and PCR assay

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12 December 2020

Real time quantitative PCR

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12 December 2020

Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

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12 December 2020

Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole

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12 December 2020

New real-time PCR able to detect in a single tube multiple rifampin resistance mutations and high-level isoniazid resistance mutations in Mycobacterium tuberculosis

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12 December 2020

A rapid real-time quantitative polymerase chain reaction for hepatitis B virus

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12 December 2020

Quantitative molecular analysis of virus expression and replication.

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12 December 2020

Sensitive and accurate quantitation of hepatitis B virus DNA using a kinetic fluorescence detection system (TaqMan PCR).

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12 December 2020

Real-time PCR technology for cancer diagnostics.

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12 December 2020

Validation of a quantitative method for real time PCR kinetics

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12 December 2020

Quantitative PCR--new diagnostic tool for quantifying specific mRNA and DNA molecules: HER2/neu DNA quantification with LightCycler real-time PCR in comparison with immunohistochemistry and fluorescence in situ hybridization.

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12 December 2020

A rapid sample preparation method for PCR detection of food pathogens based on buoyant density centrifugation

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12 December 2020

High-sensitivity quantitative PCR platform

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12 December 2020

Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis.

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12 December 2020

Quantitative RT-PCR: pitfalls and potential

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12 December 2020

Quantitation of viral DNA by real-time PCR applying duplex amplification, internal standardization, and two-color fluorescence detection

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12 December 2020

Simultaneous amplification and detection of specific DNA sequences

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12 December 2020

Detection of Australian bat lyssavirus using a fluorogenic probe.

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12 December 2020

Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method

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12 December 2020

A novel method for real time quantitative RT-PCR.

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12 December 2020

Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR.

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12 December 2020

Detection of Mycobacterium tuberculosis genotypic groups by a duplex real-time PCR targeting the katG and gyrA genes.

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12 December 2020

Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase

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12 December 2020

Quantitation of BK virus load in serum for the diagnosis of BK virus-associated nephropathy in renal transplant recipients

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12 December 2020

Housekeeping genes as internal standards: use and limits

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12 December 2020

Specificity of HercepTest in determining HER-2/neu status of breast cancers using the United States Food and Drug Administration-approved scoring system

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12 December 2020

Detection and quantification of mitochondrial DNA deletions in individual cells by real-time PCR.

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12 December 2020

HER-2 and TOP2A coamplification in urinary bladder cancer

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12 December 2020

Role of HER2/neu in tumor progression and therapy

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12 December 2020

Analysis of mixed human/microbial DNA samples: a validation study of two PCR AMP-FLP typing methods

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12 December 2020

Assessment of minimal residual disease (MRD) in CBFbeta/MYH11-positive acute myeloid leukemias by qualitative and quantitative RT-PCR amplification of fusion transcripts

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12 December 2020

Sensitivity of HER-2/neu antibodies in archival tissue samples of invasive breast carcinomas. Correlation with oncogene amplification in 160 cases

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12 December 2020

Rates of topoisomerase II-alpha and HER-2 gene amplification and expression in epithelial ovarian carcinoma.

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12 December 2020

Simultaneous detection of influenza viruses A and B using real-time quantitative PCR.

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12 December 2020

Factors affecting the performance of 5' nuclease PCR assays for Listeria monocytogenes detection

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12 December 2020

Amplification and deletion of topoisomerase IIalpha associate with ErbB-2 amplification and affect sensitivity to topoisomerase II inhibitor doxorubicin in breast cancer

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12 December 2020

Homogeneous quantitative assay of hepatitis C virus RNA by polymerase chain reaction in the presence of a fluorescent intercalater

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12 December 2020

Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis

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12 December 2020

Purification and characterization of PCR-inhibitory components in blood cells

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12 December 2020

Novel real-time quantitative PCR test for trisomy 21

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12 December 2020

Development of a one tube-one step RT-PCR protocol for the detection of seven viroids in four genera: Apscaviroid, Hostuviroid, Pelamoviroid and Pospiviroid

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12 December 2020

Fetal DNA in maternal plasma is elevated in pregnancies with aneuploid fetuses

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12 December 2020

Fluorotyping of HLA-DRB by sequence-specific priming and fluorogenic probing.

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12 December 2020

The housekeeping gene glyceraldehyde-3-phosphate dehydrogenase is inappropriate as internal control in comparative studies between skin tissue and cultured skin fibroblasts using Northern blot analysis

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12 December 2020

Standardized determination of real-time PCR efficiency from a single reaction set-up

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12 December 2020

Evaluation of real-time PCR data

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12 December 2020

Rapid and specific detection of Mycobacterium tuberculosis from acid-fast bacillus smear-positive respiratory specimens and BacT/ALERT MP culture bottles by using fluorogenic probes and real-time PCR.

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12 December 2020

Automated polymerase chain reaction in capillary tubes with hot air.

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12 December 2020

Prenatal diagnosis of congenital toxoplasmosis by duplex real-time PCR using fluorescence resonance energy transfer hybridization probes

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12 December 2020

Pitfalls of processed pseudogenes in RT-PCR

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12 December 2020

Identification of pathogenic Leptospira genospecies by continuous monitoring of fluorogenic hybridization probes during rapid-cycle PCR.

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12 December 2020

High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.

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12 December 2020

A closed tube format for amplification and detection of DNA based on energy transfer

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12 December 2020

Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data

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12 December 2020

Rapid quantitative detection of BCR-ABL transcripts in chronic myeloid leukemia patients by real-time reverse transcriptase polymerase-chain reaction using fluorescently labeled probes

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12 December 2020

Identifiers

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