Enhancing and confirming the specificity of RNAi experiments (Q36575386)

15 August 2017

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title

Enhancing and confirming the specificity of RNAi experiments (English)

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15 August 2017

Bryan R Cullen

1

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15 August 2017

1 September 2006

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15 August 2017

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15 August 2017

3

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15 August 2017

677-681

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15 August 2017

9

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https://scigraph.springernature.com/pub.10.1038/nmeth913

15 August 2017

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cites work

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Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors

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3' UTR seed matches, but not overall identity, are associated with RNAi off-targets

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Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells

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A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA.

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A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference

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RNA interference in human cells is restricted to the cytoplasm

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Induction of an interferon response by RNAi vectors in mammalian cells

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Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA

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inferred from DOI database lookup

7 January 2021

based on heuristic

1 reference

inferred from DOI database lookup

7 January 2021

based on heuristic

Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing

1 reference

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NMETH913

1 reference

15 August 2017

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PubMed ID

16929311