Making and breaking peptide bonds: protein engineering using sortase (Q37871538)

24 August 2017

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title

Making and breaking peptide bonds: protein engineering using sortase (English)

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24 August 2017

2

Hidde L Ploegh

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24 August 2017

Maximilian Wei-Lin Popp

series ordinal

1

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PubMed ID

21538739

24 August 2017

language of work or name

English

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publication date

27 April 2011

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Angewandte Chemie International Edition

24 August 2017

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volume

50

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24 August 2017

5024-5032

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24 August 2017

22

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Sortases and the art of anchoring proteins to the envelopes of gram-positive bacteria

24 August 2017

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase-catalysed anchoring of surface proteins to the cell wall of Staphylococcus aureus

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Importance of srtA and srtB for growth of Bacillus anthracis in macrophages.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Anchor structure of staphylococcal surface proteins. IV. Inhibitors of the cell wall sorting reaction

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Analysis of the substrate specificity of the Staphylococcus aureus sortase transpeptidase SrtA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sorting sortases: a nomenclature proposal for the various sortases of Gram-positive bacteria.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Identification of substrates of the Listeria monocytogenes sortases A and B by a non-gel proteomic analysis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

A comparative genome analysis identifies distinct sorting pathways in gram-positive bacteria

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Genome-wide detection and analysis of cell wall-bound proteins with LPxTG-like sorting motifs

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

An embarrassment of sortases - a richness of substrates?

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Sortase catalyzed in vitro transpeptidation reaction using LPXTG peptide and NH(2)-Gly(3) substrates.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Development of an HPLC assay for Staphylococcus aureus sortase: evidence for the formation of the kinetically competent acyl enzyme intermediate

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. III. Lipid II is an in vivo peptidoglycan substrate for sortase-catalyzed surface protein anchoring

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Assembly of pili in Gram-positive bacteria.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Pili with strong attachments: Gram-positive bacteria do it differently

21 January 2018

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https://api.crossref.org/works/10.1002%2FANIE.201008267

Pili in Gram-positive bacteria: assembly, involvement in colonization and biofilm development

21 January 2018

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https://api.crossref.org/works/10.1002%2FANIE.201008267

Pili in Gram-negative and Gram-positive bacteria - structure, assembly and their role in disease.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Assembly of pili on the surface of Corynebacterium diphtheriae.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortases and pilin elements involved in pilus assembly of Corynebacterium diphtheriae.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase-mediated pilus fiber biogenesis in Streptococcus pneumoniae

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Two crystal structures of pneumococcal pilus sortase C provide novel insights into catalysis and substrate specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase activity is controlled by a flexible lid in the pilus biogenesis mechanism of gram-positive pathogens

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Amide bonds assemble pili on the surface of bacilli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

The molecular switch that activates the cell wall anchoring step of pilus assembly in gram-positive bacteria

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Staphylococcus aureus sortase mutants defective in the display of surface proteins and in the pathogenesis of animal infections

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

The biology of Gram-positive sortase enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase as a target of anti-infective therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Recent advances towards new anti-infective agents that inhibit cell surface protein anchoring in Staphylococcus aureus and other gram-positive pathogens

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

The role of Streptococcus pneumoniae sortase A in colonisation and pathogenesis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortagging: a versatile method for protein labeling

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Site-specific protein modification on living cells catalyzed by Sortase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Assignment of the 1H, 13C and 15N signals of Sortase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Crystal structures of Staphylococcus aureus sortase A and its substrate complex.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

The structure of the Staphylococcus aureus sortase-substrate complex reveals how the universally conserved LPXTG sorting signal is recognized

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Development of a high-performance liquid chromatography assay and revision of kinetic parameters for the Staphylococcus aureus sortase transpeptidase SrtA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. A conserved arginine residue is required for efficient catalysis of sortase A.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Staphylococcus aureus Sortase A transpeptidase. Calcium promotes sorting signal binding by altering the mobility and structure of an active site loop.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Anchoring of surface proteins to the cell wall of Staphylococcus aureus. Cysteine 184 and histidine 120 of sortase form a thiolate-imidazolium ion pair for catalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase from Staphylococcus aureus does not contain a thiolate-imidazolium ion pair in its active site

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Staphylococcus aureus sortase transpeptidase SrtA: insight into the kinetic mechanism and evidence for a reverse protonation catalytic mechanism

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Kinetic Mechanism of Staphylococcus aureus Sortase SrtA

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sorting of protein a to the staphylococcal cell wall

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Establishment of an experimental system allowing immobilization of proteins on the surface of Bacillus subtilis cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

A biosynthetic strategy for re-engineering the Staphylococcus aureus cell wall with non-native small molecules.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Biological applications of protein splicing

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Synthesis of biologically active peptide nucleic acid-peptide conjugates by sortase-mediated ligation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase-mediated protein ligation: a new method for protein engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase A as a novel molecular "stapler" for sequence-specific protein conjugation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Substrate filtering by the active site crossover loop in UCHL3 revealed by sortagging and gain-of-function mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Site-specific N- and C-terminal labeling of a single polypeptide using sortases of different specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Expansion of the sortase-mediated labeling method for site-specific N-terminal labeling of cell surface proteins on living cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase-catalyzed peptide-glycosylphosphatidylinositol analogue ligation.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Sortase A-catalyzed transpeptidation of glycosylphosphatidylinositol derivatives for chemoenzymatic synthesis of GPI-anchored proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Chemoenzymatic synthesis of glycosylphosphatidylinositol-anchored glycopeptides

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Lipid modification of proteins through sortase-catalyzed transpeptidation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Enzyme-mediated site-specific antibody-protein modification using a ZZ domain as a linker

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Novel synthesis of functional mucin glycopeptides containing both N- and O-glycans

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Functional neoglycopeptides: synthesis and characterization of a new class of MUC1 glycoprotein models having core 2-based O-glycan and complex-type N-glycan chains.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Synthesis of protein mimics with nonlinear backbone topology by a combined recombinant, enzymatic, and chemical synthesis strategy

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

A straight path to circular proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Circular proteins--no end in sight

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Chemistry. Seamless proteins tie up their loose ends

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

The use of sortase-mediated ligation for the immobilisation of bacterial adhesins onto fluorescence-labelled microspheres: a novel approach to analyse bacterial adhesion to host cells.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Covalent attachment of proteins to solid supports and surfaces via Sortase-mediated ligation

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Highly oriented recombinant glycosyltransferases: site-specific immobilization of unstable membrane proteins by using Staphylococcus aureus sortase A.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

A self-cleavable sortase fusion for one-step purification of free recombinant proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Production of recombinant proteins in Escherichia coli using an N-terminal tag derived from sortase

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

A novel sortase, SrtC2, from Streptococcus pyogenes anchors a surface protein containing a QVPTGV motif to the cell wall

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Anchor structure of staphylococcal surface proteins. V. Anchor structure of the sortase B substrate IsdC.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

An iron-regulated sortase anchors a class of surface protein during Staphylococcus aureus pathogenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Surface protein IsdC and Sortase B are required for heme-iron scavenging of Bacillus anthracis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Bacillus anthracis sortase A (SrtA) anchors LPXTG motif-containing surface proteins to the cell wall envelope

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Cell wall anchor structure of BcpA pili in Bacillus anthracis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

The Sortase A Enzyme That Attaches Proteins to the Cell Wall of Bacillus anthracis Contains an Unusual Active Site Architecture

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Improved pattern for genome-based screening identifies novel cell wall-attached proteins in gram-positive bacteria

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Localization and mutagenesis of the sorting signal binding site on sortase A from Staphylococcus aureus.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Engineering the substrate specificity of Staphylococcus aureus Sortase A. The beta6/beta7 loop from SrtB confers NPQTN recognition to SrtA.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Mutagenesis studies of substrate recognition and catalysis in the sortase A transpeptidase from Staphylococcus aureus

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

Peptide−Sugar Ligation Catalyzed by Transpeptidase Sortase: A Facile Approach to Neoglycoconjugate Synthesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FANIE.201008267

21 January 2018

Identifiers

DOI

10.1002/ANIE.201008267

1 reference

24 August 2017

1 reference

PubMed ID

21538739