Fusion protein linkers: property, design and functionality (Q38048516)

27 August 2017

Fusion protein linkers: property, design and functionality (English)

1 reference

27 August 2017

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Xiaoying Chen

1

1 reference

27 August 2017

Jennica L Zaro

series ordinal

2

1 reference

27 August 2017

Wei-Chiang Shen

series ordinal

3

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27 August 2017

language of work or name

English

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publication date

29 September 2012

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Advanced Drug Delivery Reviews

27 August 2017

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27 August 2017

65

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27 August 2017

10

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27 August 2017

1357-1369

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Intramolecular regulation of the sequence-specific mRNA interferase activity of MazF fused to a MazE fragment with a linker cleavable by specific proteases

27 August 2017

1 reference

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Pharmacokinetics of recombinant bifunctional fusion proteins

6 September 2017

1 reference

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The development of romiplostim for patients with immune thrombocytopenia

6 September 2017

1 reference

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Challenges associated with the targeted delivery of gelonin to claudin-expressing cancer cells with the use of activatable cell penetrating peptides to enhance potency

6 September 2017

1 reference

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Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells

6 September 2017

1 reference

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Transferrin fusion technology: a novel approach to prolonging biological half-life of insulinotropic peptides

6 September 2017

1 reference

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Biopharmaceutical drug targeting to the brain

6 September 2017

1 reference

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Denileukin diftitox: a novel immunotoxin

6 September 2017

1 reference

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Human growth hormone-transferrin fusion protein for oral delivery in hypophysectomized rats

6 September 2017

1 reference

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Pathways and mechanisms of endocytic recycling

6 September 2017

1 reference

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Rilonacept in the treatment of chronic inflammatory disorders

6 September 2017

1 reference

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Fusion-proteins as biopharmaceuticals--applications and challenges.

6 September 2017

1 reference

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Protein therapeutics: a summary and pharmacological classification

6 September 2017

1 reference

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Cell penetrating peptides: intracellular pathways and pharmaceutical perspectives

6 September 2017

1 reference

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A flexible protein linker improves the function of epitope-tagged proteins in Saccharomyces cerevisiae

6 September 2017

1 reference

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Immunoabsorbent nanoparticles based on a tobamovirus displaying protein A.

6 September 2017

1 reference

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Improving the oral efficacy of recombinant granulocyte colony-stimulating factor and transferrin fusion protein by spacer optimization

6 September 2017

1 reference

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Monomeric Fc fusions: impact on pharmacokinetic and biological activity of protein therapeutics

6 September 2017

1 reference

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Target-mediated drug disposition and dynamics

6 September 2017

1 reference

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Fluorescence microscopy today

6 September 2017

1 reference

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Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Latent cytokines: development of novel cleavage sites and kinetic analysis of their differential sensitivity to MMP-1 and MMP-3.

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Mechanisms of TfR-mediated transcytosis and sorting in epithelial cells and applications toward drug delivery

6 September 2017

1 reference

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The crystal structure of the proprotein processing proteinase furin explains its stringent specificity

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

An analysis of protein domain linkers: their classification and role in protein folding

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Furin at the cutting edge: from protein traffic to embryogenesis and disease

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Metalloproteases and inhibitors in arthritic diseases

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Role of linkers in communication between protein modules

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Cathepsin substrates as cleavable peptide linkers in bioconjugates, selected from a fluorescence quench combinatorial library

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Expression of matrix metalloproteinases and their tissue inhibitors in human brain tumors

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

The structure and function of proline-rich regions in proteins

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Dictionary of protein secondary structure: pattern recognition of hydrogen-bonded and geometrical features

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Helix stabilization by Glu-...Lys+ salt bridges in short peptides of de novo design

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Recombinant latent transforming growth factor beta 1 has a longer plasma half-life in rats than active transforming growth factor beta 1, and a different tissue distribution

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Stereochemical quality of protein structure coordinates

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Balancing the pharmacokinetics and pharmacodynamics of interferon-α2b and human serum albumin fusion protein by proteolytic or reductive cleavage increases its in vivo therapeutic efficacy

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Design of an in vivo cleavable disulfide linker in recombinant fusion proteins

6 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Half-life extension through albumin fusion technologies

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Insertion of the designed helical linker led to increased expression of tf-based fusion proteins

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Recombinant immunoproapoptotic proteins with furin site can translocate and kill HER2-positive cancer cells

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

A flexible peptide linker enhances the immunoreactivity of two copies HBsAg preS1 (21-47) fusion protein

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Inclusion of a furin-sensitive spacer enhances the cytotoxicity of ribotoxin restrictocin containing recombinant single-chain immunotoxins

14 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Local interactions in protein folding: lessons from the alpha-helix

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Effects of receptor binding on plasma half-life of bifunctional transferrin fusion proteins

28 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Development of a long-acting insulin analog using albumin fusion technology

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

A hinge at the central helix of the regulatory light chain of myosin is critical for phosphorylation-dependent regulation of smooth muscle myosin motor activity

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Matrix metalloproteinases and diseases of the CNS.

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

The establishment of polarity and enhanced transcytosis of transferrin receptors in enterocyte-like Caco-2 cells

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

New coupling agents for the synthesis of immunotoxins containing a hindered disulfide bond with improved stability in vivo.

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Sequence-imposed structural constraints in the TonB protein of E. coli.

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

A cysteine proteinase cDNA from Trypanosoma brucei predicts an enzyme with an unusual C-terminal extension

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Production of enkephalin in tobacco protoplasts using tobacco mosaic virus RNA vector

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Molecular engineering of short half-life small peptides (VIP, αMSH and γ₃MSH) fused to latency-associated peptide results in improved anti-inflammatory therapeutics

23 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Selective cytotoxicity to HER2-positive tumor cells by a recombinant e23sFv-TD-tBID protein containing a furin cleavage sequence

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

A recombinant polypeptide extends the in vivo half-life of peptides and proteins in a tunable manner

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

In vitro and in vivo study of an albumin-binding prodrug of doxorubicin that is cleaved by cathepsin B.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Self-renaturing enzymes: design of an enzyme-chaperone chimera as a new approach to enzyme stabilization

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Increasing the homogeneity, stability and activity of human serum albumin and interferon-alpha2b fusion protein by linker engineering

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Bifunctional enhancement of a beta-glucanase-xylanase fusion enzyme by optimization of peptide linkers

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Circumventing the heterogeneity and instability of human serum albumin-interferon-alpha2b fusion protein by altering its orientation.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Conformations of variably linked chimeric proteins evaluated by synchrotron X-ray small-angle scattering.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Optimization of codon pair use within the (GGGGS)3 linker sequence results in enhanced protein expression

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Immunization with an interferon-gamma-gp120 fusion protein induces enhanced immune responses to human immunodeficiency virus gp120.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Design of the linkers which effectively separate domains of a bifunctional fusion protein.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

The ubiquitin-proteasome pathway regulates lysosomal degradation of the growth hormone receptor and its ligand

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Properties of a single-chain antibody containing different linker peptides.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Rapid protein-folding assay using green fluorescent protein

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Engineering of Functional Chimeric Protein G–VargulaLuciferase

1 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3726540

Single-chain antibodies as diagnostic tools and therapeutic agents

3 December 2018

1 reference

12 December 2020

LINKER: a program to generate linker sequences for fusion proteins

1 reference

12 December 2020

Matrix metalloproteinases: multifunctional effectors of inflammation in multiple sclerosis and bacterial meningitis

1 reference

12 December 2020

Albugranin, a recombinant human granulocyte colony stimulating factor (G-CSF) genetically fused to recombinant human albumin induces prolonged myelopoietic effects in mice and monkeys

1 reference

12 December 2020

Targeting cytokines to inflammation sites

1 reference

12 December 2020

Characterization of a long-acting recombinant human serum albumin-atrial natriuretic factor (ANF) expressed in Pichia pastoris

1 reference

12 December 2020

1H-NMR study of mobility and conformational constraints within the proline-rich N-terminal of the LC1 alkali light chain of skeletal myosin. Correlation with similar segments in other protein systems

1 reference

12 December 2020

Increase in surface expression of transferrin receptors on cultured hepatocytes of adult rats in response to iron deficiency

1 reference

12 December 2020

Single-chain antigen-binding proteins

1 reference

12 December 2020

An investigation of oligopeptides linking domains in protein tertiary structures and possible candidates for general gene fusion

1 reference

12 December 2020

Restructuring an interdomain linker in the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex of Escherichia coli

1 reference

12 December 2020

Furin activates Pseudomonas exotoxin A by specific cleavage in vivo and in vitro

1 reference

12 December 2020

Immunotoxins for targeted cancer therapy

1 reference

12 December 2020

Prolonged in-vivo half-life of factor VIIa by fusion to albumin

1 reference

12 December 2020

10.1016/J.ADDR.2012.09.039

Identifiers

DOI

1 reference

27 August 2017

1 reference

27 August 2017

PubMed publication ID

23026637

1 reference