Post-production protein stability: trouble beyond the cell factory. (Q38264351)

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21806813%20AND%20SRC:MED&resulttype=core&format=json

9 February 2020

editorial

0 references

title

Post-production protein stability: trouble beyond the cell factory (English)

1 reference

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9 February 2020

1

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9 February 2020

José Luis Corchero Nieto

2

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9 February 2020

Antonio Villaverde

3

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9 February 2020

author name string

Esther Vazquez

1

1 reference

1 September 2017

José Luis Corchero

2

1 reference

1 September 2017

Antonio Villaverde

3

1 reference

1 September 2017

1 August 2011

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21806813%20AND%20SRC:MED&resulttype=core&format=json

9 February 2020

published in

Microbial Cell Factories

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9 February 2020

volume

10

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9 February 2020

page(s)

60

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Nanoparticles: a boon to drug delivery, therapeutics, diagnostics and imaging

9 February 2020

cites work

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Translationally optimal codons associate with aggregation-prone sites in proteins

6 September 2017

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Inclusion bodies: a new concept

6 September 2017

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Cellular strategies for controlling protein aggregation

6 September 2017

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Carrier mediated protein and peptide stabilization

6 September 2017

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Isolation of cell-free bacterial inclusion bodies

6 September 2017

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Isolation of biologically active nanomaterial (inclusion bodies) from bacterial cells

6 September 2017

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Side effects of chaperone gene co-expression in recombinant protein production

6 September 2017

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The formulation and immunogenicity of therapeutic proteins: Product quality as a key factor

6 September 2017

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Nanostructured bacterial materials for innovative medicines

6 September 2017

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Quality control of inclusion bodies in Escherichia coli

6 September 2017

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Therapeutic application of peptides and proteins: parenteral forever?

6 September 2017

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Human growth hormone-transferrin fusion protein for oral delivery in hypophysectomized rats

6 September 2017

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Recent trends in stabilising peptides and proteins in pharmaceutical formulation - considerations in the choice of excipients.

6 September 2017

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E. coli transports aggregated proteins to the poles by a specific and energy-dependent process

6 September 2017

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Mutations of key hydrophobic surface residues of 11β-hydroxysteroid dehydrogenase type 1 increase solubility and monodispersity in a bacterial expression system

6 September 2017

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Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli.

6 September 2017

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Microbial factories for recombinant pharmaceuticals

6 September 2017

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Modular protein engineering in emerging cancer therapies

6 September 2017

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Use of folding modulators to improve heterologous protein production in Escherichia coli.

6 September 2017

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Engineering inclusion bodies for non denaturing extraction of functional proteins

6 September 2017

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Peptide-assisted traffic engineering for nonviral gene therapy.

6 September 2017

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Current perspectives on stability of protein drug products during formulation, fill and finish operations

6 September 2017

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Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

6 September 2017

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6 September 2017

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6 September 2017

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Protein therapeutics: a summary and pharmacological classification

6 September 2017

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6 September 2017

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6 September 2017

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Divergent genetic control of protein solubility and conformational quality in Escherichia coli

6 September 2017

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Chaperone-based procedure to increase yields of soluble recombinant proteins produced in E. coli

6 September 2017

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Genomic analysis of the secretion stress response in the enzyme-producing cell factory Aspergillus niger

6 September 2017

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Biotechnology applications of amino acids in protein purification and formulations

6 September 2017

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Current status of technical protein refolding.

6 September 2017

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Drug targeting to the brain.

6 September 2017

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Protein aggregation in crowded environments.

6 September 2017

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Protein quality in bacterial inclusion bodies.

6 September 2017

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Solubilization and refolding of bacterial inclusion body proteins

6 September 2017

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Lon and ClpP proteases participate in the physiological disintegration of bacterial inclusion bodies.

6 September 2017

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Localization of chaperones DnaK and GroEL in bacterial inclusion bodies

6 September 2017

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Protein drug stability: a formulation challenge

6 September 2017

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6 September 2017

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6 September 2017

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Advanced genetic strategies for recombinant protein expression in Escherichia coli

6 September 2017

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Recombinant protein folding and misfolding in Escherichia coli

6 September 2017

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Protein expression and refolding--a practical guide to getting the most out of inclusion bodies

6 September 2017

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Delivery of bioactive molecules into the cell: the Trojan horse approach

6 September 2017

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Stress in recombinant protein producing yeasts.

6 September 2017

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Strategies for the recovery of active proteins through refolding of bacterial inclusion body proteins

6 September 2017

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Modular protein engineering for non-viral gene therapy.

6 September 2017

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Challenges in the development of high protein concentration formulations

6 September 2017

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Protein aggregation in recombinant bacteria: biological role of inclusion bodies.

6 September 2017

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Preclinical and clinical development of immunocytokines.

6 September 2017

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Bioequivalence and the immunogenicity of biopharmaceuticals

6 September 2017

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Strategies for achieving high-level expression of genes in Escherichia coli

6 September 2017

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Binding of 125I-labeled recombinant beta interferon (IFN-beta Ser17) to human cells

6 September 2017

1 reference

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Factors influencing inclusion body formation in the production of a fused protein in Escherichia coli

6 September 2017

1 reference

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Application of a high-throughput screening procedure with PEG-induced precipitation to compare relative protein solubility during formulation development with IgG1 monoclonal antibodies

6 September 2017

1 reference

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Influence of production process design on inclusion bodies protein: the case of an Antarctic flavohemoglobin

14 September 2017

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Heterologous expression, purification, and characterization of cytochrome P450sca-2 and mutants with improved solubility in Escherichia coli

14 September 2017

1 reference

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Genetic engineering, expression, and activity of a fusion protein of a human neurotrophin and a molecular Trojan horse for delivery across the human blood-brain barrier

14 September 2017

1 reference

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Enhanced stability of recombinant keratinocyte growth factor by mutagenesis

14 September 2017

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Minimal information: an urgent need to assess the functional reliability of recombinant proteins used in biological experiments

14 September 2017

1 reference

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The biologic activity and molecular characterization of a novel synthetic interferon-alpha species, consensus interferon

27 September 2017

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Engineering building blocks for self-assembling protein nanoparticles

27 September 2017

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Nanotechnology, bionanotechnology and microbial cell factories

23 June 2018

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Rare codon content affects the solubility of recombinant proteins in a codon bias-adjusted Escherichia coli strain.

23 June 2018

1 reference

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Killing HIV-infected cells by transduction with an HIV protease-activated caspase-3 protein

23 June 2018

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Cysteine to serine substitutions in basic fibroblast growth factor: effect on inclusion body formation and proteolytic susceptibility during in vitro refolding

23 June 2018

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Influence of growth temperature on the production of antibody Fab fragments in different microbes: a host comparative analysis.

23 June 2018

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High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners

31 August 2018

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Antiepoetin antibody-related pure red cell aplasia: successful remission with cessation of recombinant erythropoietin alone.

31 August 2018

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Minimal information for protein functional evaluation (MIPFE) workshop

31 August 2018

1 reference

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Learning about protein solubility from bacterial inclusion bodies

31 August 2018

1 reference

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Effects of recombinant protein misfolding and aggregation on bacterial membranes.

31 August 2018

1 reference

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Optimization of buffer solutions for protein crystallization

31 August 2018

1 reference

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In situ protein folding and activation in bacterial inclusion bodies

31 August 2018

1 reference

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Protocol for preparing proteins with improved solubility by co-expressing with molecular chaperones in Escherichia coli.

31 August 2018

1 reference

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Addition of TAT protein transduction domain and GrpE to human p53 provides soluble fusion proteins that can be transduced into dendritic cells and elicit p53-specific T-cell responses in HLA-A*0201 transgenic mice

31 August 2018

1 reference

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Blood-brain barrier delivery of protein and non-viral gene therapeutics with molecular Trojan horses

31 August 2018

1 reference

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The conformational quality of insoluble recombinant proteins is enhanced at low growth temperatures.

31 August 2018

1 reference

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Mutational analysis of protein solubility enhancement using short peptide tags.

31 August 2018

1 reference

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Bacterial inclusion bodies are cytotoxic in vivo in absence of functional chaperones DnaK or GroEL.

31 August 2018

1 reference

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Kinetics of inclusion body formation studied in intact cells by FT-IR spectroscopy.

31 August 2018

1 reference

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Amyloid-like properties of bacterial inclusion bodies.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

The solubility and stability of recombinant proteins are increased by their fusion to NusA.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

A simple method for improving protein solubility and long-term stability.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Detection and prevention of protein aggregation before, during, and after purification.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Albumin binding as a general strategy for improving the pharmacokinetics of proteins

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Antitumor effect of TAT-oxygen-dependent degradation-caspase-3 fusion protein specifically stabilized and activated in hypoxic tumor cells.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Insulin aspart (B28 asp-insulin): a fast-acting analog of human insulin: absorption kinetics and action profile compared with regular human insulin in healthy nondiabetic subjects

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Generation of protease-deficient strains and their use in heterologous protein expression

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Nativelike secondary structure in interleukin-1 beta inclusion bodies by attenuated total reflectance FTIR.

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Polycationic amino acid tags enhance soluble expression of Candida antarctica lipase B in recombinant Escherichia coli

31 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3162505

Inclusion bodies: Specificity in their aggregation process and amyloid-like structure

1 October 2018

1 reference

Crossref

https://api.crossref.org/works/10.1186%2F1475-2859-10-60

Antibody Response to Aggregated Human Interferon Alpha2b in Wild-type and Transgenic Immune Tolerant Mice Depends on Type and Level of Aggregation

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1186%2F1475-2859-10-60

Recombinant protein solubility—does more mean better?

21 January 2018

1 reference

Crossref

https://api.crossref.org/works/10.1186%2F1475-2859-10-60

Immunogenicity of aggregates of recombinant human growth hormone in mouse models

21 January 2018

1 reference

12 December 2020

FT-IR study of heterologous protein expression in recombinant Escherichia coli strains

1 reference

12 December 2020

Protein aggregates seem to play a key role among the parameters influencing the antigenicity of interferon alpha (IFN-alpha) in normal and transgenic mice

1 reference

12 December 2020

New fusion protein systems designed to give soluble expression in Escherichia coli

1 reference

12 December 2020

Reflections on the immunogenicity of therapeutic proteins

1 reference

12 December 2020

Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy

1 reference

12 December 2020

Rapid determination of protein solubility and stability conditions for NMR studies using incomplete factorial design

1 reference

12 December 2020

Identifiers

DOI

10.1186/1475-2859-10-60

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21806813%20AND%20SRC:MED&resulttype=core&format=json

Dimensions Publication ID

9 February 2020

0 references

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:21806813%20AND%20SRC:MED&resulttype=core&format=json

9 February 2020

PubMed publication ID

21806813

1 reference