A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates (Q40443775)

20 September 2017

A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates (English)

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20 September 2017

2

Kevin A Janes

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PubMed ID

15782220

20 September 2017

Douglas A. Lauffenburger

series ordinal

3

object named as

Douglas A Lauffenburger

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20 September 2017

4

Barbara Imperiali

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20 September 2017

Melissa D Shults

1

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20 September 2017

23 March 2005

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20 September 2017

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20 September 2017

2

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20 September 2017

4

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20 September 2017

277-283

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https://scigraph.springernature.com/pub.10.1038/nmeth747

20 September 2017

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A quantitative single-cell assay for protein kinase B reveals important insights into the biochemical behavior of an intracellular substrate peptide

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Genetically encoded FRET probe for PKC activity based on pleckstrin

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Fluorescent indicators for Akt/protein kinase B and dynamics of Akt activity visualized in living cells

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A genetically encoded fluorescent reporter reveals oscillatory phosphorylation by protein kinase C

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Fluorescent indicators for imaging protein phosphorylation in single living cells

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Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells

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Genetically encoded reporters of protein kinase A activity reveal impact of substrate tethering

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A pair of fluorescent resonance energy transfer-based probes for tyrosine phosphorylation of the CrkII adaptor protein in vivo.

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Imaging of cAMP-dependent protein kinase activity in living neural cells using a novel fluorescent substrate.

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Real time visualization of protein kinase activity in living cells.

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Versatile fluorescence probes of protein kinase activity.

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Modular and tunable chemosensor scaffold for divalent zinc

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Advances in protein kinase B signalling: AKTion on multiple fronts

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PI 3-kinase, Akt and cell survival

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ERK and p38 MAPK-activated protein kinases: a family of protein kinases with diverse biological functions

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PKA: a portrait of protein kinase dynamics

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Peptide and protein library screening defines optimal substrate motifs for AKT/PKB.

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MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation

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Specificity and mechanism of action of some commonly used protein kinase inhibitors

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Role of translocation in the activation and function of protein kinase B

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Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B

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Effects of the inhibition of p38/RK MAP kinase on induction of five fos and jun genes by diverse stimuli

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Fluorometric and colorimetric detection of caspase activity associated with apoptosis

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A high-throughput quantitative multiplex kinase assay for monitoring information flow in signaling networks: application to sepsis-apoptosis

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7 January 2021

Analysis of protein kinase B/Akt

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7 January 2021

Analysis of c-Jun N-terminal kinase regulation and function

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7 January 2021

Analyzing JNK and p38 mitogen-activated protein kinase activity

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7 January 2021

Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter

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7 January 2021

A fluorescent indicator for visualizing cAMP-induced phosphorylation in vivo

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7 January 2021

Insulin activates protein kinase B, inhibits glycogen synthase kinase-3 and activates glycogen synthase by rapamycin-insensitive pathways in skeletal muscle and adipose tissue

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7 January 2021

Distinct roles for phosphoinositide 3-kinase, mitogen-activated protein kinase and p38 MAPK in mediating cell cycle progression of breast cancer cells

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7 January 2021

Direct photometric or fluorometric assay of proteinases using substrates containing 7-amino-4-trifluoromethylcoumarin

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7 January 2021

Identifiers

DOI

10.1038/NMETH747

1 reference

PubMed ID

15782220

Dimensions Publication ID

20 September 2017

0 references

1 reference

PubMed ID

15782220