Recombinant insect cell expression and purification of human beta-secretase (BACE-1) for X-ray crystallography (Q40629788)

22 September 2017

title

Recombinant insect cell expression and purification of human beta-secretase (BACE-1) for X-ray crystallography (English)

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22 September 2017

main subject

X-ray crystallography

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author name string

Wouter Bruinzeel

1

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22 September 2017

Jeff Yon

2

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22 September 2017

Silvio Giovannelli

3

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22 September 2017

Stefan Masure

4

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22 September 2017

publication date

1 October 2002

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Protein Expression and Purification

22 September 2017

published in

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22 September 2017

volume

26

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22 September 2017

issue

1

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22 September 2017

page(s)

139-148

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The cell biology of beta-amyloid precursor protein and presenilin in Alzheimer's disease

22 September 2017

cites work

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Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE

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Purification and cloning of amyloid precursor protein beta-secretase from human brain

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Identification of a Novel Aspartic Protease (Asp 2) as β-Secretase

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Human aspartic protease memapsin 2 cleaves the beta-secretase site of beta-amyloid precursor protein

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A new BACE is for therapy of Alzheimer's disease

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Design of Potent Inhibitors for Human Brain Memapsin 2 (β-Secretase)

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Structure of the protease domain of memapsin 2 (beta-secretase) complexed with inhibitor

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Characterization of the glycosylation profiles of Alzheimer's beta -secretase protein Asp-2 expressed in a variety of cell lines.

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Characterization of recombinant, soluble beta-secretase from an insect cell expression system.

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Maturation and endosomal targeting of beta-site amyloid precursor protein-cleaving enzyme. The Alzheimer's disease beta-secretase

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Characterization of Alzheimer's beta -secretase protein BACE. A pepsin family member with unusual properties

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Substrate and inhibitor profile of BACE (beta-secretase) and comparison with other mammalian aspartic proteases

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Proteolytic activation of recombinant pro-memapsin 2 (pro-beta-secretase) studied with new fluorogenic substrates

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Post-translational processing of beta-secretase (beta-amyloid-converting enzyme) and its ectodomain shedding. The pro- and transmembrane/cytosolic domains affect its cellular activity and amyloid-beta production

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The pro domain of beta-secretase does not confer strict zymogen-like properties but does assist proper folding of the protease domain

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A furin-like convertase mediates propeptide cleavage of BACE, the Alzheimer's beta -secretase

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Processing of beta-secretase by furin and other members of the proprotein convertase family

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Assembly of SIV virus-like particles containing envelope proteins using a baculovirus expression system

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7 January 2021

10.1016/S1046-5928(02)00516-8

Identifiers

DOI

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22 September 2017

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