Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system (Q41647084)

6 October 2017

Production of bispecific antibodies in "knobs-into-holes" using a cell-free expression system (English)

1 reference

6 October 2017

Yiren Xu

1

1 reference

6 October 2017

John Lee

2

1 reference

6 October 2017

Cuong Tran

3

1 reference

6 October 2017

Tyler H Heibeck

4

1 reference

6 October 2017

Willie D Wang

5

1 reference

6 October 2017

Junhao Yang

6

1 reference

6 October 2017

Ryan L Stafford

7

1 reference

6 October 2017

Alexander R Steiner

8

1 reference

6 October 2017

Aaron K Sato

9

1 reference

6 October 2017

Trevor J Hallam

10

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6 October 2017

Gang Yin

11

1 reference

6 October 2017

1 January 2015

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6 October 2017

Monoclonal Antibodies

1 reference

6 October 2017

7

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6 October 2017

1

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6 October 2017

231-242

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Creative Commons Attribution-NonCommercial 3.0 Unported

6 October 2017

start time

2 January 2015

1 reference

April 2022 Public Data File from Crossref

copyright status

copyrighted

1 reference

April 2022 Public Data File from Crossref

cites work

Antitumor efficacy of a bispecific antibody that targets HER2 and activates T cells

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Structural design of disialoganglioside GD2 and CD3-bispecific antibodies to redirect T cells for tumor therapy

6 June 2018

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Nanobody-based products as research and diagnostic tools

6 June 2018

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Antiparallel conformation of knob and hole aglycosylated half-antibody homodimers is mediated by a CH2-CH3 hydrophobic interaction

6 June 2018

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Engineering toward a bacterial "endoplasmic reticulum" for the rapid expression of immunoglobulin proteins

6 June 2018

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Generation of bispecific IgG antibodies by structure-based design of an orthogonal Fab interface

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Production of site-specific antibody-drug conjugates using optimized non-natural amino acids in a cell-free expression system

6 June 2018

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Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially broadly protective influenza vaccines

6 June 2018

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A tale of two specificities: bispecific antibodies for therapeutic and diagnostic applications

6 June 2018

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Antitumor immunity: easy as 1, 2, 3 with monoclonal bispecific trifunctional antibodies?

6 June 2018

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Targeting T cells to tumor cells using bispecific antibodies

6 June 2018

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Cell-free translation of peptides and proteins: from high throughput screening to clinical production

6 June 2018

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Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange.

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Domain order of a bispecific diabody dramatically enhances its antitumor activity beyond structural format conversion: the case of the hEx3 diabody

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Progress in overcoming the chain association issue in bispecific heterodimeric IgG antibodies.

6 June 2018

1 reference

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Advances in bispecific biotherapeutics for the treatment of cancer

6 June 2018

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Generating bispecific human IgG1 and IgG2 antibodies from any antibody pair

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

scFv antibody: principles and clinical application

6 June 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Dual targeting strategies with bispecific antibodies

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Aglycosylated antibodies and antibody fragments produced in a scalable in vitro transcription-translation system

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Protein targeting constructs in alpha therapy

6 June 2018

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Emerging technologies for making glycan-defined glycoproteins

6 June 2018

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A novel bispecific antibody format enables simultaneous bivalent and monovalent co-engagement of distinct target antigens

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

A two-in-one antibody against HER3 and EGFR has superior inhibitory activity compared with monospecific antibodies

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Immunoglobulin domain crossover as a generic approach for the production of bispecific IgG antibodies

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Chemical generation of bispecific antibodies

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Stable IgG-like bispecific antibodies directed toward the type I insulin-like growth factor receptor demonstrate enhanced ligand blockade and anti-tumor activity

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Development of a two-part strategy to identify a therapeutic human bispecific antibody that inhibits IgE receptor signaling

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Enhancing antibody Fc heterodimer formation through electrostatic steering effects: applications to bispecific molecules and monovalent IgG.

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Effector cell recruitment with novel Fv-based dual-affinity re-targeting protein leads to potent tumor cytolysis and in vivo B-cell depletion

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Introducing antigen-binding sites in structural loops of immunoglobulin constant domains: Fc fragments with engineered HER2/neu-binding sites and antibody properties

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Aglycosylated IgG variants expressed in bacteria that selectively bind FcgammaRI potentiate tumor cell killing by monocyte-dendritic cells

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

A modular IgG-scFv bispecific antibody topology

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Strategies to extend plasma half-lives of recombinant antibodies

6 June 2018

1 reference

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Glycosylation as a strategy to improve antibody-based therapeutics

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Glycoprotein synthesis: an update.

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Aglycosylated immunoglobulin G1 variants productively engage activating Fc receptors

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Multifunctional antibodies by the Dock-and-Lock method for improved cancer imaging and therapy by pretargeting

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

The dock and lock method: a novel platform technology for building multivalent, multifunctional structures of defined composition with retained bioactivity

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Effect of linker sequences between the antibody variable domains on the formation, stability and biological activity of a bispecific tandem diabody

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Design, Construction, and In Vitro Analyses of Multivalent Antibodies

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Bispecific human IgG by design

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Bispecific tandem diabody for tumor therapy with improved antigen binding and pharmacokinetics

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

An efficient route to human bispecific IgG.

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Stable heterodimers from remodeling the domain interface of a homodimer using a phage display library

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

"Diabodies": small bivalent and bispecific antibody fragments

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Cell-free synthesis of the H-cluster: a model for the in vitro assembly of metalloprotein metal centers.

6 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

A prokaryote-based cell-free translation system that efficiently synthesizes glycoproteins.

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Efficient disulfide bond formation in virus-like particles

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

SEEDbodies: fusion proteins based on strand-exchange engineered domain (SEED) CH3 heterodimers in an Fc analogue platform for asymmetric binders or immunofusions and bispecific antibodies

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Improving the productivity of single-chain Fv antibody against c-Met by rearranging the order of its variable domains.

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

High yield cell-free production of integral membrane proteins without refolding or detergents

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Chemical production of bispecific antibodies

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Enhancing multiple disulfide bonded protein folding in a cell-free system

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Effect of domain order on the activity of bacterially produced bispecific single-chain Fv antibodies.

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

A new model for intermediate molecular weight recombinant bispecific and trispecific antibodies by efficient heterodimerization of single chain variable domains through fusion to a Fab-chain

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Design and production of novel tetravalent bispecific antibodies

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

An improved linker for single-chain Fv with reduced aggregation and enhanced proteolytic stability.

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4623329

Isolation and characterization of IgG1 with asymmetrical Fc glycosylation

18 August 2018

1 reference

12 December 2020

A “Tag-and-Modify” Approach to Site-Selective Protein Modification

1 reference

12 December 2020

EpCAM/CD3-Bispecific T-cell engaging antibody MT110 eliminates primary human pancreatic cancer stem cells

1 reference

12 December 2020

'Knobs-into-holes' engineering of antibody CH3 domains for heavy chain heterodimerization

1 reference

12 December 2020

Improved tumour targeting by disulphide stabilized diabodies expressed in Pichia pastoris

1 reference

12 December 2020

Simultaneous activation of T cells and accessory cells by a new class of intact bispecific antibody results in efficient tumor cell killing

1 reference

12 December 2020

Design and generation of DVD-Ig™ molecules for dual-specific targeting

1 reference

12 December 2020

10.4161/19420862.2015.989013

Identifiers

DOI

1 reference

6 October 2017

1 reference

6 October 2017

PubMed publication ID

25427258

1 reference