Site-specific biotinylation of purified proteins using BirA. (Q41874378)

13 October 2017

Site-specific biotinylation of purified proteins using BirA. (English)

1 reference

13 October 2017

1

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

author name string

Mark Howarth

series ordinal

2

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

language of work or name

English

0 references

publication date

1 January 2015

1 reference

Methods in Molecular Biology

13 October 2017

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

volume

1266

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13 June 2020

page(s)

171-184

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json

Imaging trans-cellular neurexin-neuroligin interactions by enzymatic probe ligation

13 June 2020

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Cholesterol loading and ultrastable protein interactions determine the level of tumor marker required for optimal isolation of cancer cells

5 June 2018

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Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity

5 June 2018

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DOLORS: versatile strategy for internal labeling and domain localization in electron microscopy

5 June 2018

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Ideal, catch, and slip bonds in cadherin adhesion

5 June 2018

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Engineered ascorbate peroxidase as a genetically encoded reporter for electron microscopy

5 June 2018

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Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources

5 June 2018

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Ultrafast protein splicing is common among cyanobacterial split inteins: implications for protein engineering

5 June 2018

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Flagellar hook flexibility is essential for bundle formation in swimming Escherichia coli cells.

5 June 2018

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A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

5 June 2018

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Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin

5 June 2018

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Expression and purification of E. coli BirA biotin ligase for in vitro biotinylation

5 June 2018

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Cell-type-specific nuclei purification from whole animals for genome-wide expression and chromatin profiling.

5 June 2018

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A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axonal transport

5 June 2018

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How the biotin–streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer

5 June 2018

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MHC-peptide tetramers for the analysis of antigen-specific T cells

5 June 2018

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A fluorophore ligase for site-specific protein labeling inside living cells

5 June 2018

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A streptavidin variant with slower biotin dissociation and increased mechanostability

5 June 2018

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A native chromatin purification system for epigenomic profiling in Caenorhabditis elegans

5 June 2018

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Designed auto-assembly of nanostreptabodies for rapid tissue-specific targeting in vivo.

5 June 2018

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Histone H2A.Z and DNA methylation are mutually antagonistic chromatin marks

5 June 2018

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Tags for labeling protein N-termini with subtiligase for proteomics

5 June 2018

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An artificial processivity clamp made with streptavidin facilitates oriented attachment of polymerase-DNA complexes to surfaces

5 June 2018

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Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform

5 June 2018

1 reference

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Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin

5 June 2018

1 reference

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Expanding the substrate tolerance of biotin ligase through exploration of enzymes from diverse species

5 June 2018

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Sortagging: a versatile method for protein labeling

5 June 2018

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Membrane lateral diffusion and capture of CFTR within transient confinement zones.

5 June 2018

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A monovalent streptavidin with a single femtomolar biotin binding site

5 June 2018

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Targeting quantum dots to surface proteins in living cells with biotin ligase

5 June 2018

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Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase

5 June 2018

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Site-directed, Ligase-Independent Mutagenesis (SLIM): a single-tube methodology approaching 100% efficiency in 4 h.

5 June 2018

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In vivo biotinylation of the major histocompatibility complex (MHC) class II/peptide complex by coexpression of BirA enzyme for the generation of MHC class II/tetramers

5 June 2018

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Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice

5 June 2018

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Intein-mediated biotinylation of proteins and its application in a protein microarray

5 June 2018

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Metabolic biotinylation of secreted and cell surface proteins from mammalian cells

5 June 2018

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Biotinylation of proteins in vivo and in vitro using small peptide tags

5 June 2018

1 reference

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In vivo enzymatic protein biotinylation

5 June 2018

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A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation

5 June 2018

1 reference

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Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins

5 June 2018

1 reference

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Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli.

5 June 2018

1 reference

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The INTACT method for cell type-specific gene expression and chromatin profiling in Arabidopsis thaliana.

5 June 2018

1 reference

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Targeted and proximity-dependent promiscuous protein biotinylation by a mutant Escherichia coli biotin protein ligase

18 August 2018

1 reference

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Development of an enzymatic method for site-specific incorporation of desthiobiotin to recombinant proteins in vitro

18 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4304673

Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins

18 August 2018

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12 December 2020

Avidin and streptavidin

1 reference

12 December 2020

Genetic engineering of streptavidin, a versatile affinity tag

1 reference

12 December 2020

In vitro enzymatic biotinylation of recombinant fab fragments through a peptide acceptor tail

1 reference

12 December 2020

BirA enzyme: production and application in the study of membrane receptor-ligand interactions by site-specific biotinylation

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12 December 2020

Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system

1 reference

12 December 2020

Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties

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12 December 2020

Generation of epitope-tagged proteins by inverse polymerase chain reaction mutagenesis

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12 December 2020

Stable, high-affinity streptavidin monomer for protein labeling and monovalent biotin detection

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12 December 2020

10.1007/978-1-4939-2272-7_12

Identifiers

DOI

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

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https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json

13 June 2020

PubMed publication ID

25560075

1 reference

https://www.ebi.ac.uk/europepmc/webservices/rest/search?query=EXT_ID:25560075%20AND%20SRC:MED&resulttype=core&format=json