PCR-amplification of GC-rich regions: 'slowdown PCR'. (Q46421359)

22 December 2017

title

PCR-amplification of GC-rich regions: 'slowdown PCR'. (English)

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22 December 2017

author name string

Ulrich H Frey

1

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22 December 2017

Hagen S Bachmann

2

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22 December 2017

Jürgen Peters

3

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22 December 2017

Winfried Siffert

4

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22 December 2017

publication date

1 January 2008

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22 December 2017

published in

Nature Protocols

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22 December 2017

volume

3

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22 December 2017

issue

8

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22 December 2017

page(s)

1312-1317

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22 December 2017

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

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Optimization and troubleshooting in PCR

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inferred from DOI database lookup

7 January 2021

based on heuristic

High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR.

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2008.112

inferred from DOI database lookup

7 January 2021

based on heuristic

Identifiers

DOI

10.1038/NPROT.2008.112

1 reference

22 December 2017

1 reference

22 December 2017