Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution (Q30751852)

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English	Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution	scientific article

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scholarly article

1 reference

Europe PubMed Central

23 September 2017

1 reference

Europe PubMed Central

Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution (English)

1 reference

Europe PubMed Central

23 September 2017

directed evolution

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protein evolution

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1

1 reference

Europe PubMed Central

26 June 2017

language of work or name

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publication date

27 February 2004

1 reference

Europe PubMed Central

23 September 2017

Nucleic Acids Research

1 reference

Europe PubMed Central

23 September 2017

32

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Europe PubMed Central

23 September 2017

4

1 reference

Europe PubMed Central

23 September 2017

1448-1459

1 reference

Europe PubMed Central

23 September 2017

Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Directed evolution of a bacterial α‐amylase: Toward enhanced pH‐performance and higher specific activity

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Removing the redundancy from randomised gene libraries.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Library analysis of SCHEMA-guided protein recombination

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

User-friendly algorithms for estimating completeness and diversity in randomized protein-encoding libraries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Preparation of SCRATCHY hybrid protein libraries: size- and in-frame selection of nucleic acid sequences

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

The creation of ITCHY hybrid protein libraries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Saturation mutagenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Production of randomly mutated plasmid libraries using mutator strains

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Creating random mutagenesis libraries by megaprimer PCR of whole plasmid (MEGAWHOP).

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Generating mutant libraries using error-prone PCR.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

General method for sequence-independent site-directed chimeragenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: the role of alanine 230 in acceptor subsite +1

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Significantly enhanced stability of glucose dehydrogenase by directed evolution.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Forced Evolution of a Herbicide Detoxifying Glutathione Transferase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Directed evolution of N-acetylneuraminic acid aldolase to catalyze enantiomeric aldol reactions

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

A universal, vector-based system for nucleic acid reading-frame selection

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Genetic screens and directed evolution for protein solubility

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Random DNA fragmentation with endonuclease V: application to DNA shuffling

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Milestones in directed enzyme evolution.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Screening and selection methods for large-scale analysis of protein function

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Deracemization of alpha-methylbenzylamine using an enzyme obtained by in vitro evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Structure-based combinatorial protein engineering (SCOPE).

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Protein building blocks preserved by recombination

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

eCodonOpt: a systematic computational framework for optimizing codon usage in directed evolution experiments

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Analysis of shuffled gene libraries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Directed enzyme evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Investigating and Engineering Enzymes by Genetic Selection

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Creating multiple-crossover DNA libraries independent of sequence identity

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

DNA shuffling method for generating highly recombined genes and evolved enzymes

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Predicting crossover generation in DNA shuffling.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Large-scale solid-phase preparation of 3'-unprotected trinucleotide phosphotriesters--precursors for synthesis of trinucleotide phosphoramidites

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Orthogonal combinatorial mutagenesis: a codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

In vitro evolution of beta-glucuronidase into a beta-galactosidase proceeds through non-specific intermediates

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

A new randomization assay reveals unexpected elements of sequence bias in model 'randomized' gene libraries: implications for biopanning

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Patterned library analysis: a method for the quantitative assessment of hypotheses concerning the determinants of protein structure.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Exploring nonnatural evolutionary pathways by saturation mutagenesis: rapid improvement of protein function

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

A combinatorial approach to hybrid enzymes independent of DNA homology

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Directed evolution of an esterase: screening of enzyme libraries based on pH-indicators and a growth assay

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Molecular evolution by staggered extension process (StEP) in vitro recombination

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Combinatorial library diversity: probability assessment of library populations

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

A convenient approach to the synthesis of trinucleotide phosphoramidites--synthons for the generation of oligonucleotide/peptide libraries

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Rapid evolution of a protein in vitro by DNA shuffling

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

Antibody engineering by codon-based mutagenesis in a filamentous phage vector system.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

20 June 2018

A comparison of directed evolution approaches using the beta-glucuronidase model system.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Staggered extension process (StEP) in vitro recombination.

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Mutations in distant residues moderately increase the enantioselectivity of Pseudomonas fluorescens esterase towards methyl 3bromo-2-methylpropanoate and ethyl 3phenylbutyrate

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Directed evolution of a glutaryl acylase into an adipyl acylase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Directed evolution of an enantioselective lipase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

The effect of high-frequency random mutagenesis on in vitro protein evolution: a study on TEM-1 beta-lactamase

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=390300

25 November 2018

RACHITT: Gene family shuffling by Random Chimeragenesis on Transient Templates

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Combination of DMT-mononucleotide and Fmoc-trinucleotide phosphoramidites in oligonucleotide synthesis affords an automatable codon-level mutagenesis method

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Creating randomized amino acid libraries with the QuikChange Multi Site-Directed Mutagenesis Kit

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Family shuffling with single-stranded DNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Random insertion and deletion mutagenesis

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Novel family shuffling methods for the in vitro evolution of enzymes

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Creating random mutagenesis libraries using megaprimer PCR of whole plasmid

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

An effective family shuffling method using single-stranded DNA

1 reference

https://pubmed.ncbi.nlm.nih.gov/14990750

12 December 2020

based on heuristic

inferred from PubMed ID database lookup

Identifiers

10.1093/NAR/GKH315

1 reference

Europe PubMed Central

23 September 2017

1 reference

Europe PubMed Central

23 September 2017

1 reference

Europe PubMed Central

23 September 2017

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