General method for sequence-independent site-directed chimeragenesis (Q30951889)

27 June 2017

General method for sequence-independent site-directed chimeragenesis (English)

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27 June 2017

2

Frances H Arnold

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27 June 2017

Kaori Hiraga

1

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PubMed ID

12823968

27 June 2017

language of work or name

English

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publication date

1 July 2003

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Journal of Molecular Biology

27 June 2017

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27 June 2017

330

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27 June 2017

2

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27 June 2017

287-296

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Protein evolution by molecular breeding

27 June 2017

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

DNA shuffling method for generating highly recombined genes and evolved enzymes

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

DNA shuffling of a family of genes from diverse species accelerates directed evolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Random-priming in vitro recombination: an effective tool for directed evolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Rapid evolution of a protein in vitro by DNA shuffling

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Molecular evolution by staggered extension process (StEP) in vitro recombination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Analysis of shuffled gene libraries

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Finding Cinderella's slipper--proteins that fit

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Creating multiple-crossover DNA libraries independent of sequence identity

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Libraries of hybrid proteins from distantly related sequences

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Domain combinations in archaeal, eubacterial and eukaryotic proteomes

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Introns and gene evolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

The multiplicity of domains in proteins

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Protein folds, functions and evolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Structure-based combinatorial protein engineering (SCOPE).

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Random multi-recombinant PCR for the construction of combinatorial protein libraries

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Directed evolution of proteins by exon shuffling

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Foldability of barnase mutants obtained by permutation of modules or secondary structure units

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Constructing high complexity synthetic libraries of long ORFs using in vitro selection

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Novel folded protein domains generated by combinatorial shuffling of polypeptide segments

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Crystal structure of Escherichia coli TEM1 beta-lactamase at 1.8 A resolution

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Insights into the molecular basis for the carbenicillinase activity of PSE-4 beta-lactamase from crystallographic and kinetic studies

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

BaeI, another unusual BcgI-like restriction endonuclease

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Library analysis of SCHEMA-guided protein recombination

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

Characterization of a PSE-4 mutant with different properties in relation to penicillanic acid sulfones: importance of residues 216 to 218 in class A beta-lactamases

7 January 2021

1 reference

https://api.crossref.org/works/10.1016%2FS0022-2836%2803%2900590-4

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7 January 2021

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Identifiers

DOI

10.1016/S0022-2836(03)00590-4

1 reference

27 June 2017

1 reference

PubMed ID

12823968