Construction and flow cytometric screening of targeted enzyme libraries (Q33455423)

26 July 2017

title

Construction and flow cytometric screening of targeted enzyme libraries (English)

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26 July 2017

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1

Navin Varadarajan

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26 July 2017

Jason R Cantor

2

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26 July 2017

George Georgiou

series ordinal

3

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26 July 2017

Brent L Iverson

series ordinal

4

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26 July 2017

publication date

21 May 2009

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26 July 2017

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26 July 2017

volume

4

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26 July 2017

issue

6

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26 July 2017

page(s)

893-901

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Saturation mutagenesis of putative catalytic residues of benzoylformate decarboxylase provides a challenge to the accepted mechanism

26 July 2017

cites work

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Directed evolution: an approach to engineer enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Design and evolution of new catalytic activity with an existing protein scaffold

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Kemp elimination catalysts by computational enzyme design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Furilisin: a variant of subtilisin BPN' engineered for cleaving tribasic substrates.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Evolving strategies for enzyme engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Evolution of highly active enzymes by homology-independent recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Improved biocatalysts by directed evolution and rational protein design

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Enzyme promiscuity: evolutionary and mechanistic aspects.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Highly active and selective endopeptidases with programmed substrate specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Improving enzyme properties: when are closer mutations better?

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Synthetic antibody libraries focused towards peptide ligands.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Recent progress in biocatalyst discovery and optimization

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Antibody engineering by parsimonious mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

An efficient system for the evolution of aminoacyl-tRNA synthetase specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Protein engineering with bacterial display

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Touchdown PCR for increased specificity and sensitivity in PCR amplification

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

21 January 2018

1 reference

https://api.crossref.org/works/10.1038%2FNPROT.2009.60

Error-prone polymerase chain reaction for modification of scFvs

21 January 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/19478805

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1038/NPROT.2009.60

1 reference

Dimensions Publication ID

26 July 2017

1009742489

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