Directed evolution: an approach to engineer enzymes (Q33254710)

24 July 2017

1 reference

title

Directed evolution: an approach to engineer enzymes (English)

1 reference

24 July 2017

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Jasjeet Kaur

1

1 reference

PubMed ID

16923533

24 July 2017

Rohit Sharma

series ordinal

2

1 reference

24 July 2017

1 July 2006

1 reference

Critical Reviews in Biotechnology

24 July 2017

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24 July 2017

26

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24 July 2017

3

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24 July 2017

165-199

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High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

24 July 2017

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Structural basis of selection and thermostability of laboratory evolved Bacillus subtilis lipase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of new catalytic activity using the alpha/beta-barrel scaffold

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Alteration of substrate specificity of aspartase by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Engineering the substrate specificity of cytochrome P450 CYP102A2 by directed evolution: production of an efficient enzyme for bioconversion of fine chemicals.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

On the origin of genomic adaptation at high temperature for prokaryotic organisms

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Engineering of pyranose 2-oxidase from Peniophora gigantea towards improved thermostability and catalytic efficiency

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Evolving strategies for enzyme engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Biodegradation of 1,2,3-trichloropropane through directed evolution and heterologous expression of a haloalkane dehalogenase gene

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of toluene ortho-monooxygenase for enhanced 1-naphthol synthesis and chlorinated ethene degradation

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Bacterial cell surface display of organophosphorus hydrolase for selective screening of improved hydrolysis of organophosphate nerve agents

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of ATP binding proteins from a zinc finger domain by using mRNA display

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Narrowing substrate specificity in a directly evolved enzyme: the A293D mutant of aspartate aminotransferase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Global incorporation of norleucine in place of methionine in cytochrome P450 BM-3 heme domain increases peroxygenase activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Alcohol-mediated error-prone PCR.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

DNA shuffling method for generating highly recombined genes and evolved enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Promiscuous methylation of non-canonical DNA sites by HaeIII methyltransferase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Enzymes from extremophiles

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

In vitro selection of restriction endonucleases by in vitro compartmentalization

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution and substrate specificity profile of homing endonuclease I-SceI.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

New enzymes for biotransformations

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Protein engineering of toluene 4-monooxygenase of Pseudomonas mendocina KR1 for synthesizing 4-nitrocatechol from nitrobenzene

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Biphenyl dioxygenases: functional versatilities and directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Orthogonal combinatorial mutagenesis: a codon-level combinatorial mutagenesis method useful for low multiplicity and amino acid-scanning protocols

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of polymerase function by compartmentalized self-replication

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Generic expansion of the substrate spectrum of a DNA polymerase by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of an extremely fast phosphotriesterase by in vitro compartmentalization

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Forced evolution of glutathione S-transferase to create a more efficient drug detoxication enzyme.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Engineering p-hydroxyphenylpyruvate dioxygenase to a p-hydroxymandelate synthase and evidence for the proposed benzene oxide intermediate in homogentisate formation

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Isolation of novel ribozymes that ligate AMP-activated RNA substrates

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Metabolic engineering of bacteria for environmental applications: construction of Pseudomonas strains for biodegradation of 2-chlorotoluene

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

General method for sequence-independent site-directed chimeragenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Changing the substrate specificity of cytochrome c peroxidase using directed evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Whole organism biocatalysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of enantioselective enzymes for organic chemistry

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Enantioselective biocatalysis optimized by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution and the creation of enantioselective biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Analysis of shuffled gene libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of Thermus maltogenic amylase toward enhanced thermal resistance

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Industrial enzyme applications

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Enhanced degradation of polychlorinated biphenyls by directed evolution of biphenyl dioxygenase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of mammalian cytochrome P450 2B1: mutations outside of the active site enhance the metabolism of several substrates, including the anticancer prodrugs cyclophosphamide and ifosfamide

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of Escherichia coli farnesyl diphosphate synthase (IspA) reveals novel structural determinants of chain length specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Investigating the target recognition of DNA cytosine-5 methyltransferase HhaI by library selection using in vitro compartmentalisation

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Conversion of cyclodextrin glycosyltransferase into a starch hydrolase by directed evolution: the role of alanine 230 in acceptor subsite +1

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Catalytic asymmetric vinylation and dienylation of ketones

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Functional expression of horseradish peroxidase in E. coli by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Improving the carboligase activity of benzoylformate decarboxylase from Pseudomonas putida by a combination of directed evolution and site-directed mutagenesis

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Alteration of the substrate specificity of benzoylformate decarboxylase from Pseudomonas putida by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Rapid generation of incremental truncation libraries for protein engineering using alpha-phosphothioate nucleotides.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Creating multiple-crossover DNA libraries independent of sequence identity

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Inverting enantioselectivity by directed evolution of hydantoinase for improved production of L-methionine

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Growth of Escherichia coli coexpressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Library analysis of SCHEMA-guided protein recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

In vitro evolution and characterization of a ligase ribozyme adapted to acidic conditions: effect of further rounds of evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of a para-nitrobenzyl esterase for aqueous-organic solvents

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Functional expression of horseradish peroxidase in Saccharomyces cerevisiae and Pichia pastoris

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Functional expression and stabilization of horseradish peroxidase by directed evolution in Saccharomyces cerevisiae

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Mutations of penicillin acylase residue B71 extend substrate specificity by decreasing steric constraints for substrate binding

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Structure-based combinatorial protein engineering (SCOPE).

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Improvement of oxidative and thermostability of N-carbamyl-d-amino Acid amidohydrolase by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Diversification and specialization of HIV protease function during in vitro evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Predicting the emergence of antibiotic resistance by directed evolution and structural analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Theoretical distribution of truncation lengths in incremental truncation libraries.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Incremental truncation as a strategy in the engineering of novel biocatalysts

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

A combinatorial approach to hybrid enzymes independent of DNA homology

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Preparation of human metabolites of propranolol using laboratory-evolved bacterial cytochromes P450.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Altering the substrate specificity of cephalosporin acylase by directed evolution of the Beta -subunit.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of RuBisCO hypermorphs through genetic selection in engineered E.coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Regio- and enantioselective alkane hydroxylation with engineered cytochromes P450 BM-3.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Designed evolution of enzymatic properties

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Application of the "codon-shuffling" method. Synthesis and selection of de novo proteins as antibacterials

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

REBASE: restriction enzymes and methyltransferases

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution relieves product inhibition and confers in vivo function to a rationally designed tyrosine aminotransferase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Active site engineering of the epoxide hydrolase from Agrobacterium radiobacter AD1 to enhance aerobic mineralization of cis-1,2-dichloroethylene in cells expressing an evolved toluene ortho-monooxygenase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Metabolic pathway engineering to enhance aerobic degradation of chlorinated ethenes and to reduce their toxicity by cloning a novel glutathione S-transferase, an evolved toluene o-monooxygenase, and gamma-glutamylcysteine synthetase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Different strategies to recover the activity of monomeric triosephosphate isomerase by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Laboratory evolution of toluene dioxygenase to accept 4-picoline as a substrate

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Thermostabilization of a cytochrome p450 peroxygenase.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of the site specificity of Cre recombinase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution to increase camptothecin sensitivity of human DNA topoisomerase I.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of a recombinase for improved genomic integration at a native human sequence

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Laboratory evolution of cytochrome p450 BM-3 monooxygenase for organic cosolvents

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Libraries of hybrid proteins from distantly related sequences

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Discovery of pectin-degrading enzymes and directed evolution of a novel pectate lyase for processing cotton fabric

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of Thermotoga neapolitana xylose isomerase: high activity on glucose at low temperature and low pH

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Rapid evolution of a protein in vitro by DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Man-made cell-like compartments for molecular evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of enzymes for product chemistry.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Simultaneous optimization of enzyme activity and quaternary structure by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Increased amylosucrase activity and specificity, and identification of regions important for activity, specificity and stability through molecular evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Enantioselective organocatalytic intramolecular Diels-Alder reactions. The asymmetric synthesis of solanapyrone D.

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of novel polymerase activities: mutation of a DNA polymerase into an efficient RNA polymerase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of extradiol dioxygenase by a novel in vivo DNA shuffling

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

New understandings of thermostable and peizostable enzymes

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of an aspartate aminotransferase with new substrate specificities

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of subtilisin E in Bacillus subtilis to enhance total activity in aqueous dimethylformamide

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

An approach to random mutagenesis of DNA using mixtures of triphosphate derivatives of nucleoside analogues

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

21 January 2018

Industrial biocatalysis

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Assembly of designed oligonucleotides as an efficient method for gene recombination: a new tool in directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Improving tolerance of Candida antarctica lipase B towards irreversible thermal inactivation through directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Generation of novel-substrate-accepting biphenyl dioxygenases through segmental random mutagenesis and identification of residues involved in enzyme specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

A general model of error-prone PCR

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Laboratory-evolved vanadium chloroperoxidase exhibits 100-fold higher halogenating activity at alkaline pH: catalytic effects from first and second coordination sphere mutations

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

Enhancement of the activity of l-aspartase from Escherichia coli W by directed evolution

21 January 2018

1 reference

https://api.crossref.org/works/10.1080%2F07388550600851423

10.1080/07388550600851423

21 January 2018

Identifiers

DOI

1 reference

24 July 2017

1 reference

PubMed ID

16923533