siRNAs: applications in functional genomics and potential as therapeutics (Q34310668)

1 August 2017

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title

siRNAs: applications in functional genomics and potential as therapeutics (English)

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1 August 2017

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2

Thomas Tuschl

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1 August 2017

Yair Dorsett

1

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1 August 2017

1 April 2004

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Nature Reviews Drug Discovery

1 August 2017

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1 August 2017

3

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1 August 2017

4

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1 August 2017

318-329

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https://scigraph.springernature.com/pub.10.1038/nrd1345

1 August 2017

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cites work

RNA interference is mediated by 21- and 22-nucleotide RNAs

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A species of small antisense RNA in posttranscriptional gene silencing in plants

31 March 2020

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Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

31 March 2020

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RNA as a target of double-stranded RNA-mediated genetic interference in Caenorhabditis elegans

31 March 2020

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Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA

31 March 2020

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Use of dsRNA-mediated genetic interference to demonstrate that frizzled and frizzled 2 act in the wingless pathway.

31 March 2020

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Double-stranded RNA induces mRNA degradation in Trypanosoma brucei

31 March 2020

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Homology-dependent gene silencing in plants and fungi: a number of variations on the same theme

31 March 2020

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How cells respond to interferons

31 March 2020

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An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells

31 March 2020

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RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals

31 March 2020

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Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate

31 March 2020

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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

31 March 2020

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Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems

31 March 2020

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Phenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses

31 March 2020

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Approaches for the sequence-specific knockdown of mRNA.

31 March 2020

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Novel antisense and peptide nucleic acid strategies for controlling gene expression

31 March 2020

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Morpholino oligos: making sense of antisense?

31 March 2020

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RNA cleaving '10-23' DNAzymes with enhanced stability and activity

31 March 2020

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Inhibition of HIV-1 gene expression by novel DNA enzymes targeted to cleave HIV-1 TAR RNA: potential effectiveness against all HIV-1 isolates

31 March 2020

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A general purpose RNA-cleaving DNA enzyme

31 March 2020

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Inhibiting expression of specific genes in mammalian cells with 5' end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA.

31 March 2020

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Antisense technologies. Improvement through novel chemical modifications

31 March 2020

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2'-O-(2-Methoxy)ethyl-modified anti-intercellular adhesion molecule 1 (ICAM-1) oligonucleotides selectively increase the ICAM-1 mRNA level and inhibit formation of the ICAM-1 translation initiation complex in human umbilical vein endothelial cells.

31 March 2020

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Functional amounts of dystrophin produced by skipping the mutated exon in the mdx dystrophic mouse

31 March 2020

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Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities

31 March 2020

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The chemical repertoire of natural ribozymes

31 March 2020

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Cleavage of an inaccessible site by the maxizyme with two independent binding arms: an alternative approach to the recruitment of RNA helicases.

31 March 2020

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Intracellular applications of ribozymes.

31 March 2020

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Expression of small, therapeutic RNAs in human cell nuclei.

31 March 2020

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Role for a bidentate ribonuclease in the initiation step of RNA interference

31 March 2020

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Single-stranded antisense siRNAs guide target RNA cleavage in RNAi

31 March 2020

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RNA interference in human cells is restricted to the cytoplasm

31 March 2020

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A model for RNA-mediated gene silencing in higher plants.

31 March 2020

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Transcriptional silencing and promoter methylation triggered by double-stranded RNA

31 March 2020

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De novo methylation and co-suppression induced by a cytoplasmically replicating plant RNA virus

31 March 2020

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Replicating satellite RNA induces sequence-specific DNA methylation and truncated transcripts in plants

31 March 2020

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Endogenous and silencing-associated small RNAs in plants

31 March 2020

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Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi

31 March 2020

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Small RNAs correspond to centromere heterochromatic repeats

31 March 2020

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Establishment and maintenance of a heterochromatin domain

31 March 2020

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RNAi-mediated targeting of heterochromatin by the RITS complex

31 March 2020

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31 March 2020

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Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery

31 March 2020

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Role of microRNAs in plant and animal development

31 March 2020

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MicroRNAs: genomics, biogenesis, mechanism, and function

31 March 2020

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MicroRNA maturation: stepwise processing and subcellular localization

31 March 2020

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The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation

31 March 2020

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Two genetic circuits repress the Caenorhabditis elegans heterochronic gene lin-28 after translation initiation

31 March 2020

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Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans

31 March 2020

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miRNP:mRNA association in polyribosomes in a human neuronal cell line.

31 March 2020

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Identification of many microRNAs that copurify with polyribosomes in mammalian neurons

31 March 2020

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A microRNA in a multiple-turnover RNAi enzyme complex

31 March 2020

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Independent combinatorial effect of antisense oligonucleotides and RNAi-mediated specific inhibition of the recombinant rat P2X3 receptor

31 March 2020

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Comparison of the Suppressive Effects of Antisense Oligonucleotides and siRNAs Directed Against the Same Targets in Mammalian Cells

31 March 2020

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The activity of siRNA in mammalian cells is related to structural target accessibility: a comparison with antisense oligonucleotides

31 March 2020

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Comparison of different antisense strategies in mammalian cells using locked nucleic acids, 2'-O-methyl RNA, phosphorothioates and small interfering RNA.

31 March 2020

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Effective small interfering RNAs and phosphorothioate antisense DNAs have different preferences for target sites in the luciferase mRNAs

31 March 2020

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Efficient reduction of target RNAs by small interfering RNA and RNase H-dependent antisense agents. A comparative analysis

31 March 2020

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Comparison of antisense oligonucleotides and siRNAs in cell culture and in vivo.

31 March 2020

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Asymmetry in the assembly of the RNAi enzyme complex

31 March 2020

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Functional siRNAs and miRNAs Exhibit Strand Bias

31 March 2020

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Sequence, chemical, and structural variation of small interfering RNAs and short hairpin RNAs and the effect on mammalian gene silencing

31 March 2020

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Efficient and isoform-selective inhibition of cellular gene expression by peptide nucleic acids

31 March 2020

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Phosphorothioate oligodeoxynucleotides: what is their origin and what is unique about them?

31 March 2020

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siRNA-based inhibition specific for mutant SOD1 with single nucleotide alternation in familial ALS, compared with ribozyme and DNA enzyme

31 March 2020

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Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells

31 March 2020

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RNA hairpin loops repress protein synthesis more strongly than hammerhead ribozymes

31 March 2020

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Antisense oligonucleotides: promise and reality.

31 March 2020

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Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants.

31 March 2020

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Antisense and sensibility

31 March 2020

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Protein enhancement of hammerhead ribozyme catalysis

31 March 2020

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Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8

31 March 2020

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Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA

31 March 2020

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Expression profiling reveals off-target gene regulation by RNAi

31 March 2020

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Genomewide view of gene silencing by small interfering RNAs

31 March 2020

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Specificity of short interfering RNA determined through gene expression signatures

31 March 2020

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Nonspecific, concentration-dependent stimulation and repression of mammalian gene expression by small interfering RNAs (siRNAs).

31 March 2020

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Activation of the interferon system by short-interfering RNAs

31 March 2020

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Induction of an interferon response by RNAi vectors in mammalian cells

31 March 2020

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Increased specificity for antisense oligodeoxynucleotide targeting of RNA cleavage by RNase H using chimeric methylphosphonodiester/phosphodiester structures

31 March 2020

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Small RNAs with imperfect match to endogenous mRNA repress translation. Implications for off-target activity of small inhibitory RNA in mammalian cells

31 March 2020

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siRNAs can function as miRNAs

31 March 2020

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MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms

31 March 2020

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31 March 2020

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Allele-specific silencing of dominant disease genes

31 March 2020

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Selective silencing by RNAi of a dominant allele that causes amyotrophic lateral sclerosis

31 March 2020

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Allele-specific silencing of a pathogenic mutant acetylcholine receptor subunit by RNA interference

31 March 2020

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Toward therapy for DYT1 dystonia: allele-specific silencing of mutant TorsinA

31 March 2020

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Expanding small RNA interference

31 March 2020

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siRNA-mediated gene silencing in vitro and in vivo.

31 March 2020

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Adenoviral vectors expressing siRNAs for discovery and validation of gene function

31 March 2020

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Gene silencing by adenovirus-delivered siRNA.

31 March 2020

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Specific gene inhibition by adenovirus-mediated expression of small interfering RNA

31 March 2020

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Use of adeno-associated viral vector for delivery of small interfering RNA.

31 March 2020

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Local gene knockdown in the brain using viral-mediated RNA interference.

31 March 2020

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Stable suppression of tumorigenicity by virus-mediated RNA interference

31 March 2020

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Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference

31 March 2020

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A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference

31 March 2020

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Efficient lentiviral vectors for short hairpin RNA delivery into human cells

31 March 2020

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Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector

31 March 2020

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Inducible shRNA expression for application in a prostate cancer mouse model

31 March 2020

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Establishment of conditional vectors for hairpin siRNA knockdowns

31 March 2020

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Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter.

31 March 2020

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Electroporation and RNA interference in the rodent retina in vivo and in vitro

31 March 2020

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Spatial and temporal 'knock down' of gene expression by electroporation of double-stranded RNA and morpholinos into early postimplantation mouse embryos

31 March 2020

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RNAi reveals doublecortin is required for radial migration in rat neocortex

31 March 2020

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Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA

31 March 2020

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Gene silencing by systemic delivery of synthetic siRNAs in adult mice

31 March 2020

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Inhibition of synapse assembly in mammalian muscle in vivo by RNA interference

31 March 2020

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RNA interference in adult mice.

31 March 2020

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Efficient delivery of siRNA for inhibition of gene expression in postnatal mice

31 March 2020

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Small interfering RNA Inhibits Hepatitis B virus replication in mice

31 March 2020

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Inhibition of hepatitis B virus replication in vivo by nucleoside analogues and siRNA.

31 March 2020

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Caspase 8 small interfering RNA prevents acute liver failure in mice

31 March 2020

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RNA interference targeting Fas protects mice from fulminant hepatitis

31 March 2020

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Inhibition of hepatitis B virus in mice by RNA interference

31 March 2020

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Emerging clinical applications of RNA

31 March 2020

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Dissecting HIV-1 through RNA interference

31 March 2020

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Nucleic-acid therapeutics: basic principles and recent applications

31 March 2020

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The role of readthrough acetylcholinesterase in the pathophysiology of myasthenia gravis.

31 March 2020

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Success for oral antisense therapy

31 March 2020

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Discovery of transdermal penetration enhancers by high-throughput screening

31 March 2020

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Enhanced gene expression in mouse lung after PEI-DNA aerosol delivery

31 March 2020

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Germline transmission of RNAi in mice

31 March 2020

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An epi-allelic series of p53 hypomorphs created by stable RNAi produces distinct tumor phenotypes in vivo

31 March 2020

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Development of an siRNA-based method for repressing specific genes in renal organ culture and its use to show that the Wt1 tumour suppressor is required for nephron differentiation.

31 March 2020

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Systematic genome-wide screens of gene function

31 March 2020

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Systematic functional analysis of the Caenorhabditis elegans genome using RNAi

31 March 2020

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Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes

31 March 2020

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Functional genomic analysis of C. elegans chromosome I by systematic RNA interference

31 March 2020

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Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III

31 March 2020

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Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi

31 March 2020

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Gene clustering based on RNAi phenotypes of ovary-enriched genes in C. elegans

31 March 2020

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RNAi analysis of genes expressed in the ovary of Caenorhabditis elegans

31 March 2020

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Genome-wide RNAi of C. elegans using the hypersensitive rrf-3 strain reveals novel gene functions

31 March 2020

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A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans

31 March 2020

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Identification of genes that protect the C. elegans genome against mutations by genome-wide RNAi

31 March 2020

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A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity

31 March 2020

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Identification of Hedgehog pathway components by RNAi in Drosophila cultured cells.

31 March 2020

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A functional genomic analysis of cell morphology using RNA interference

31 March 2020

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Genome-wide RNAi analysis of growth and viability in Drosophila cells

31 March 2020

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Random mutagenesis screen for dominant behavioral mutations in mice

31 March 2020

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A systematic, genome-wide, phenotype-driven mutagenesis programme for gene function studies in the mouse

31 March 2020

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Genome-wide, large-scale production of mutant mice by ENU mutagenesis.

31 March 2020

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Functional genetic analysis of mouse chromosome 11

31 March 2020

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Too much interference: injection of double-stranded RNA has nonspecific effects in the zebrafish embryo

31 March 2020

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Vertebrate microRNA genes

31 March 2020

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Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA

31 March 2020

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Screening for gene function in chicken embryo using RNAi and electroporation.

31 March 2020

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Transgenic RNAi in mouse oocytes: a simple and fast approach to study gene function.

31 March 2020

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Identification of Modulators of TRAIL-Induced Apoptosis via RNAi-Based Phenotypic Screening

31 March 2020

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An approach to genomewide screens of expressed small interfering RNAs in mammalian cells.

31 March 2020

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Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-kappaB

31 March 2020

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The effectiveness of double-stranded short inhibitory RNAs (siRNAs) may depend on the method of transfection

31 March 2020

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Targeting the kinesin Eg5 to monitor siRNA transfection in mammalian cells

31 March 2020

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Identification of NF-kappa B-regulated genes induced by TNFalpha utilizing expression profiling and RNA interference

31 March 2020

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Microarrays of cells expressing defined cDNAs

31 March 2020

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Applications of transfected cell microarrays in high-throughput drug discovery.

31 March 2020

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RNAi microarray analysis in cultured mammalian cells

31 March 2020

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Rational siRNA design for RNA interference

31 March 2020

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Enhancement of RNAi activity by improved siRNA duplexes.

31 March 2020

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Free energy lights the path toward more effective RNAi

31 March 2020

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Analysis of gene function in somatic mammalian cells using small interfering RNAs

31 March 2020

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Enzymatic production of RNAi libraries from cDNAs.

31 March 2020

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Restriction enzyme-generated siRNA (REGS) vectors and libraries

31 March 2020

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siRNAs generated by recombinant human Dicer induce specific and significant but target site-independent gene silencing in human cells

31 March 2020

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Unlocking the money-making potential of RNAi.

31 March 2020

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Sustained small interfering RNA-mediated human immunodeficiency virus type 1 inhibition in primary macrophages

31 March 2020

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Silencing of H-ras gene expression by retrovirus-mediated siRNA decreases transformation efficiency and tumorgrowth in a model of human ovarian cancer

31 March 2020

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In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by e6 siRNA

31 March 2020

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Kinetic Characterization of Ribonuclease-Resistant 2′-Modified Hammerhead Ribozymes

31 March 2020

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Chemical modification of hammerhead ribozymes. Catalytic activity and nuclease resistance

31 March 2020

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A review of the issues in the pharmacokinetics and toxicology of phosphorothioate antisense oligonucleotides

31 March 2020

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Structural variations and stabilising modifications of synthetic siRNAs in mammalian cells

31 March 2020

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siRNA function in RNAi: a chemical modification analysis

31 March 2020

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Tolerance for mutations and chemical modifications in a siRNA.

31 March 2020

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RNA interference in mammalian cells by chemically-modified RNA.

31 March 2020

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RNAi in human cells: basic structural and functional features of small interfering RNA.

31 March 2020

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Positional effects of short interfering RNAs targeting the human coagulation trigger Tissue Factor

31 March 2020

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31 March 2020

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Inhibition of HIV-1 infection by small interfering RNA-mediated RNA interference

31 March 2020

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31 March 2020

Identifiers

DOI

10.1038/NRD1345

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1 August 2017

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PubMed ID

15060527