Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens (Q34547509)

25 December 2017

title

Perturb-Seq: Dissecting Molecular Circuits with Scalable Single-Cell RNA Profiling of Pooled Genetic Screens (English)

1 reference

25 December 2017

single cell transcriptomics

main subject

RNA sequencing

0 references

1 reference

A curated database reveals trends in single-cell transcriptomics

7 February 2021

15

1 reference

25 December 2017

Jenny Chen

Jenny Chen

4

0 references

Jonathan Weissman

14

Jonathan S Weissman

1 reference

25 December 2017

Danielle Dionne

8

Danielle Dionne

1 reference

25 December 2017

Charles P Fulco

5

Charles P Fulco

1 reference

25 December 2017

author name string

Atray Dixit

1

1 reference

25 December 2017

Oren Parnas

2

1 reference

25 December 2017

Biyu Li

3

1 reference

25 December 2017

Livnat Jerby-Arnon

6

1 reference

25 December 2017

Nemanja D Marjanovic

7

1 reference

25 December 2017

Tyler Burks

9

1 reference

25 December 2017

Britt Adamson

11

1 reference

25 December 2017

Thomas M Norman

12

1 reference

25 December 2017

language of work or name

English

0 references

publication date

1 December 2016

1 reference

25 December 2017

external data available at URL

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE90063

1 reference

A curated database reveals trends in single-cell transcriptomics

7 February 2021

1 reference

25 December 2017

volume

167

1 reference

25 December 2017

issue

7

1 reference

25 December 2017

page(s)

1853-1866.e17

1 reference

25 December 2017

describes a project that uses

cell culture

1 reference

A curated database reveals trends in single-cell transcriptomics

7 February 2021

Perturb-seq

1 reference

A curated database reveals trends in single-cell transcriptomics

A Multiplexed Single-Cell CRISPR Screening Platform Enables Systematic Dissection of the Unfolded Protein Response

7 February 2021

cites work

1 reference

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Multiplex gene editing by CRISPR-Cpf1 using a single crRNA array

7 July 2018

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A global genetic interaction network maps a wiring diagram of cellular function

7 July 2018

1 reference

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Comprehensive Classification of Retinal Bipolar Neurons by Single-Cell Transcriptomics

7 July 2018

1 reference

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Low Dimensionality in Gene Expression Data Enables the Accurate Extraction of Transcriptional Programs from Shallow Sequencing

7 July 2018

1 reference

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Highly multiplexed simultaneous detection of RNAs and proteins in single cells

7 July 2018

1 reference

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The Signal Transducer and Activator of Transcription 1 (STAT1) Inhibits Mitochondrial Biogenesis in Liver and Fatty Acid Oxidation in Adipocytes

7 July 2018

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Identification and characterization of essential genes in the human genome.

7 July 2018

1 reference

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ATF3 Is a Key Regulator of Macrophage IFN Responses

7 July 2018

1 reference

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A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks

7 July 2018

1 reference

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Signal transducer and activator of transcription 2 deficiency is a novel disorder of mitochondrial fission

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

GM-CSF Mouse Bone Marrow Cultures Comprise a Heterogeneous Population of CD11c(+)MHCII(+) Macrophages and Dendritic Cells.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells.

7 July 2018

1 reference

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Brain structure. Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq

7 July 2018

1 reference

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Expression profiling. Combinatorial labeling of single cells for gene expression cytometry

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Computational analysis of cell-to-cell heterogeneity in single-cell RNA-sequencing data reveals hidden subpopulations of cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Statistical significance of variables driving systematic variation in high-dimensional data

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

CRISPR-Cas9 knockin mice for genome editing and cancer modeling

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

GABP transcription factor (nuclear respiratory factor 2) is required for mitochondrial biogenesis

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Single-cell RNA-seq reveals dynamic paracrine control of cellular variation

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Toward a new STATe: the role of STATs in mitochondrial function

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Single-cell transcriptomics reveals bimodality in expression and splicing in immune cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Multiplex genome engineering using CRISPR/Cas systems

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Compensatory dendritic cell development mediated by BATF-IRF interactions

7 July 2018

1 reference

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A high-throughput chromatin immunoprecipitation approach reveals principles of dynamic gene regulation in mammals

7 July 2018

1 reference

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STAT proteins - key regulators of anti-viral responses, inflammation, and tumorigenesis in the liver

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Systematic discovery of TLR signaling components delineates viral-sensing circuits

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Metabolic labeling of RNA uncovers principles of RNA production and degradation dynamics in mammalian cells

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

The NF-kappaB p50:p50:HDAC-1 repressor complex orchestrates transcriptional inhibition of multiple pro-inflammatory genes

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Phenotypic profiling of the human genome by time-lapse microscopy reveals cell division genes

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Unbiased reconstruction of a mammalian transcriptional network mediating pathogen responses

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Cytokine-mediated increases in fetal hemoglobin are associated with globin gene histone modification and transcription factor reprogramming.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Bioinformatic analysis reveals cRel as a regulator of a subset of interferon-stimulated genes

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Maps of random walks on complex networks reveal community structure

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Histone deacetylase 3 down-regulates cholesterol synthesis through repression of lanosterol synthase gene expression

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Distinct modes of action applied by transcription factors STAT1 and IRF1 to initiate transcription of the IFN-gamma-inducible gbp2 gene.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

The Connectivity Map: using gene-expression signatures to connect small molecules, genes, and disease

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Systems biology approaches identify ATF3 as a negative regulator of Toll-like receptor 4.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Regulation and co-ordination of nuclear gene expression during mitochondrial biogenesis

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Functional discovery via a compendium of expression profiles

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Test of synergistic interactions among deleterious mutations in bacteria

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Identification of cDC1- and cDC2-committed DC progenitors reveals early lineage priming at the common DC progenitor stage in the bone marrow.

7 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Large-scale genetic perturbations reveal regulatory networks and an abundance of gene-specific repressors.

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping.

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Structure and function of a transcriptional network activated by the MAPK Hog1.

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Revealing modular organization in the yeast transcriptional network.

27 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=5181115

Modular combinatorial binding among human trans-acting factors reveals direct and indirect factor binding

27 October 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/27984732

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Massively parallel digital transcriptional profiling of single cells

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/27984732

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1016/J.CELL.2016.11.038

1 reference

25 December 2017

PMCID

5181115

1 reference

25 December 2017

PubMed ID

27984732

1 reference

25 December 2017