Positional effects of fusion partners on the yield and solubility of MBP fusion proteins (Q35371470)

8 August 2017

title

Positional effects of fusion partners on the yield and solubility of MBP fusion proteins (English)

1 reference

8 August 2017

author name string

Sreejith Raran-Kurussi

series ordinal

1

1 reference

8 August 2017

Karina Keefe

series ordinal

2

1 reference

8 August 2017

David S Waugh

series ordinal

3

1 reference

8 August 2017

publication date

14 March 2015

1 reference

Protein Expression and Purification

8 August 2017

published in

1 reference

8 August 2017

volume

110

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8 August 2017

page(s)

159-164

1 reference

Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system

8 August 2017

cites work

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

Overproduction, purification and structure determination of human dual-specificity phosphatase 14

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

Mutations that alter the equilibrium between open and closed conformations of Escherichia coli maltose-binding protein impede its ability to enhance the solubility of passenger proteins

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

Enhancement of soluble protein expression through the use of fusion tags

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers

27 July 2018

1 reference

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The P1' specificity of tobacco etch virus protease

27 July 2018

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Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency

27 July 2018

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Single amino acid substitutions on the surface of Escherichia coli maltose-binding protein can have a profound impact on the solubility of fusion proteins

27 July 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4393804

Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused

27 July 2018

1 reference

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Expression, purification, and physicochemical characterization of a recombinant Yersinia protein tyrosine phosphatase

27 July 2018

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Expression of proteins in Escherichia coli as fusions with maltose-binding protein to rescue non-expressed targets in a high-throughput protein-expression and purification pipeline

27 July 2018

1 reference

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Incorporating a TEV cleavage site reduces the solubility of nine recombinant mouse proteins

26 September 2018

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Escherichia coli fusion carrier proteins act as solubilizing agents for recombinant uncoupling protein 1 through interactions with GroEL.

26 September 2018

1 reference

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Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression

26 September 2018

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High-Resolution Structure of the Yersinia pestis Protein Tyrosine Phosphatase YopH in Complex with a Phosphotyrosyl Mimetic-Containing Hexapeptide

26 September 2018

1 reference

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Formation of soluble inclusion bodies by hpv e6 oncoprotein fused to maltose-binding protein

26 September 2018

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25782741

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Solubility-enhancing proteins MBP and NusA play a passive role in the folding of their fusion partners

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25782741

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Order of fusions between bacterial and mammalian proteins can determine solubility in Escherichia coli

1 reference

PubMed

https://pubmed.ncbi.nlm.nih.gov/25782741

inferred from PubMed ID database lookup

12 December 2020

based on heuristic

Identifiers

DOI

10.1016/J.PEP.2015.03.004

1 reference

8 August 2017

1 reference

8 August 2017

1 reference