High-throughput screening assays for the identification of chemical probes (Q36885398)

16 August 2017

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title

High-throughput screening assays for the identification of chemical probes (English)

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16 August 2017

5

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16 August 2017

6

Christopher P Austin

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16 August 2017

3

Anton Simeonov

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16 August 2017

7

Douglas S Auld

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16 August 2017

4

Menghang Xia

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16 August 2017

James Inglese

1

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16 August 2017

Ronald L Johnson

series ordinal

2

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16 August 2017

language of work or name

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1 August 2007

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16 August 2017

published in

Nature Chemical Biology

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16 August 2017

volume

3

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16 August 2017

issue

8

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16 August 2017

page(s)

466-479

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A continuous spectrophotometric assay for the hepatitis C virus serine protease

16 August 2017

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Fluorescence technologies for the investigation of chemical libraries

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Quantum dot bioconjugates for imaging, labelling and sensing

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High-content screening moves to the front of the line

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Application of laser-scanning fluorescence microplate cytometry in high content screening

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Structural basis for the spectral difference in luciferase bioluminescence.

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A novel HIV-1 antiviral high throughput screening approach for the discovery of HIV-1 inhibitors.

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Development of a Novel Dicistronic Reporter-Selectable Hepatitis C Virus Replicon Suitable for High-Throughput Inhibitor Screening

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7 January 2021

Fusions to beta-lactamase as a reporter for gene expression in live mammalian cells

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7 January 2021

Development of an intact cell reporter gene beta-lactamase assay for G protein-coupled receptors for high-throughput screening

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7 January 2021

High-throughput screening using beta-lactamase reporter-gene technology for identification of low-molecular-weight antagonists of the human gonadotropin releasing hormone receptor

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7 January 2021

Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET).

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7 January 2021

Application of scintillation proximity assay in drug discovery

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7 January 2021

Miniaturization of absorbance assays using the fluorescent properties of white microplates

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7 January 2021

Therapeutic targets: progress of their exploration and investigation of their characteristics.

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7 January 2021

High-throughput screening: update on practices and success

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7 January 2021

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

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7 January 2021

Protein phosphorylation in signaling--50 years and counting

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identification of kinase inhibitors by an ATP depletion method

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identification of small molecule ceramide kinase inhibitors using a homogeneous chemiluminescence high throughput assay

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Transcreener: screening enzymes involved in covalent regulation

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A generic, homogenous method for measuring kinase and inhibitor activity via adenosine 5'-diphosphate accumulation

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Immobilized metal ion affinity-based fluorescence polarization (IMAP): advances in kinase screening

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development and validation of a fluorescence technology for both primary and secondary screening of kinases that facilitates compound selectivity and site-specific inhibitor determination

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identification of inhibitors of the kinase activity of oncogenic V600E BRAF in an enzyme cascade high-throughput screen

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

GPCR screening via ERK 1/2: a novel platform for screening G protein-coupled receptors

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Beta galactosidase enzyme fragment complementation as a novel technology for high throughput screening

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Enzyme fragment complementation binding assay for p38alpha mitogen-activated protein kinase to study the binding kinetics of enzyme inhibitors

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identification and characterization of pleckstrin-homology-domain-dependent and isoenzyme-specific Akt inhibitors

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Protease inhibitors: current status and future prospects

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development of Novel Assays for Proteolytic Enzymes Using Rhodamine-Based Fluorogenic Substrates

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Miniaturizable homogenous time-resolved fluorescence assay for carboxypeptidase B activity

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development and application of a GFP-FRET intracellular caspase assay for drug screening

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A collaborative screening program for the discovery of inhibitors of HCV NS2/3 cis-cleaving protease activity

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development of a cell-based assay for monitoring specific hepatitis C virus NS3/4A protease activity in mammalian cells

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Novel functional assay for proteases and modulators. Application in beta-secretase studies

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Nuclear receptor minireview series

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Gain-of-function somatic cell lines for drug discovery applications generated by homologous recombination

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Assay development and data analysis of receptor-ligand binding based on scintillation proximity assay

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development of high throughput screening assays using fluorescence polarization: nuclear receptor-ligand-binding and kinase/phosphatase assays

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A homogeneous cell-based assay to measure nuclear translocation using beta-galactosidase enzyme fragment complementation.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A glucocorticoid/retinoic acid receptor chimera that displays cytoplasmic/nuclear translocation in response to retinoic acid. A real time sensing assay for nuclear receptor ligands

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Enzymatic detection of protein translocation

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development of a coactivator displacement assay for the orphan receptor estrogen-related receptor-gamma using time-resolved fluorescence resonance energy transfer

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Correlation of high-throughput pregnane X receptor (PXR) transactivation and binding assays

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Emerging concepts of guanine nucleotide-binding protein-coupled receptor (GPCR) function and implications for high throughput screening

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

The 7 TM G-protein-coupled receptor target family

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https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Real-time monitoring of morphological changes in living cells by electronic cell sensor arrays: an approach to study G protein-coupled receptors

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Lanthanide-based luminescent assays for ligand-receptor interactions.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Inverse, protean, and ligand-selective agonism: matters of receptor conformation

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

The development of an europium-GTP assay to quantitate chemokine antagonist interactions for CXCR4 and CCR5.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Measuring intracellular calcium fluxes in high throughput mode

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

cAMP detection methods in HTS: selecting the best from the rest

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

High-throughput real-time monitoring of Gs-coupled receptor activation in intact cells using cyclic nucleotide-gated channels

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

CypHer 5: a generic approach for measuring the activation and trafficking of G protein-coupled receptors in live cells

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Monitoring agonist-promoted conformational changes of beta-arrestin in living cells by intramolecular BRET.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Biosensing of opioids using frog melanophores

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Integrative functional assays, chemical genomics and high throughput screening: harnessing signal transduction pathways to a common HTS readout

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Galpha(16/z) chimeras efficiently link a wide range of G protein-coupled receptors to calcium mobilization

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Is Galpha16 the optimal tool for fishing ligands of orphan G-protein-coupled receptors?

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

D-myo-inositol 1-phosphate as a surrogate of D-myo-inositol 1,4,5-tris phosphate to monitor G protein-coupled receptor activation.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

High-throughput screening of G protein-coupled receptor antagonists using a bioluminescence resonance energy transfer 1-based beta-arrestin2 recruitment assay

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

High throughput assay technologies for ion channel drug discovery.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Nonradioactive rubidium ion efflux assay and its applications in drug discovery and development

1 reference

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7 January 2021

Population patch clamp improves data consistency and success rates in the measurement of ionic currents

1 reference

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7 January 2021

HERG-Lite: a novel comprehensive high-throughput screen for drug-induced hERG risk

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Comparison of in vitro P-glycoprotein screening assays: recommendations for their use in drug discovery

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A high-throughput cell-based assay for inhibitors of ABCG2 activity

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Characterisation of mammalian GLUT glucose transporters in a heterologous yeast expression system

1 reference

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7 January 2021

Drug discovery in academia

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Targeting the Hedgehog pathway in cancer

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Reporter gene imaging of protein-protein interactions in living subjects

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identifying off-target effects and hidden phenotypes of drugs in human cells

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Evaluation of the InteraX system technology in a high-throughput screening environment

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Imaging protein molecules using FRET and FLIM microscopy

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A miniaturized cell-based fluorescence resonance energy transfer assay for insulin-receptor activation.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Automated high content screening for phosphoinositide 3 kinase inhibition using an AKT 1 redistribution assay.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Nuclear export inhibitors and kinase inhibitors identified using a MAPK-activated protein kinase 2 redistribution screen

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identification of RAS-mitogen-activated protein kinase signaling pathway modulators in an ERF1 redistribution screen

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Image-based screening for the identification of novel proteasome inhibitors

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Losartan, an AT1 antagonist, prevents aortic aneurysm in a mouse model of Marfan syndrome

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Diverse small-molecule modulators of SMN expression found by high-throughput compound screening: early leads towards a therapeutic for spinal muscular atrophy.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

PTC124 targets genetic disorders caused by nonsense mutations

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Pharmacological chaperones: potential treatment for conformational diseases.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Nuclear antisense effects of neutral, anionic and cationic oligonucleotide analogs

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A potent small molecule inhibits polyglutamine aggregation in Huntington's disease neurons and suppresses neurodegeneration in vivo

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Predicting therapeutic value in the lead optimization phase of drug discovery

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Comparison of 3 AT1 receptor binding assays: filtration assay, ScreenReady Target, and WGA Flashplate

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Comparison of Assay Technologies for a Nuclear Receptor Assay Screen Reveals Differences in the Sets of Identified Functional Antagonists

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Mechanistic considerations in high-throughput screening

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Further concerns over Cheng-Prusoff analysis

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Determining appropriate substrate conversion for enzymatic assays in high-throughput screening

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A fluorescence polarization assay for the identification of inhibitors of the p53-DM2 protein-protein interaction

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development of a binding assay for p53/HDM2 by using homogeneous time-resolved fluorescence.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Ligand-receptor binding measured by laser-scanning imaging

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

1,4-Benzodiazepine-2,5-diones as small molecule antagonists of the HDM2-p53 interaction: discovery and SAR.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Engaging science and technology for drug development

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A simple cell-based HTS assay system to screen for inhibitors of p53-Hdm2 protein-protein interactions

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Development of a high-throughput screening assay for inhibitors of aggrecan cleavage using luminescent oxygen channeling (AlphaScreen )

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Substrate capacity considerations in developing kinase assays

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Inhibition profiling of human carbonic anhydrase II by high-throughput screening of structurally diverse, biologically active compounds

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Miniaturization of a hepatitis C virus RNA polymerase assay using a -102 degrees C cooled CCD camera-based imaging system

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Nonleadlikeness and leadlikeness in biochemical screening

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A continuous time-resolved fluorescence assay for identification of BACE1 inhibitors.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

The Application of Fluorescence Lifetime Readouts in High-Throughput Screening

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

A high-throughput screen for aggregation-based inhibition in a large compound library

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Resveratrol inhibits firefly luciferase

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence.

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

The use of bioluminescence resonance energy transfer for the study of therapeutic targets: application to tyrosine kinase receptors

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Quantitation of transcription and clonal selection of single living cells with beta-lactamase as reporter

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Fluorescent-conjugated polymer superquenching facilitates highly sensitive detection of proteases

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

High throughput ion-channel pharmacology: planar-array-based voltage clamp

1 reference

https://api.crossref.org/works/10.1038%2FNCHEMBIO.2007.17

7 January 2021

Identifiers

DOI

10.1038/NCHEMBIO.2007.17

1 reference

16 August 2017

1 reference