Update on in vitro cytotoxicity assays for drug development (Q44748416)

5 December 2017

Update on in vitro cytotoxicity assays for drug development (English)

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5 December 2017

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Andrew L Niles

1

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5 December 2017

Richard A Moravec

series ordinal

2

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5 December 2017

Terry L Riss

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3

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5 December 2017

language of work or name

English

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publication date

1 June 2008

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Expert Opinion on Drug Discovery

5 December 2017

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5 December 2017

3

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5 December 2017

6

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5 December 2017

655-669

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Direct measurement of cell numbers in microtitre plate cultures using the fluorescent dye SYBR green I.

5 December 2017

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Viability measurements in mammalian cell systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Toxicity determined in vitro by morphological alterations and neutral red absorption

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Four deaths and a funeral: from caspases to alternative mechanisms.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Is cell death a critical end point for anticancer therapies or is cytostasis sufficient?

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Calculating the probability of detection for inhibitors in enzymatic or binding reactions in high-throughput screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Quantitative high-throughput screening: a titration-based approach that efficiently identifies biological activities in large chemical libraries

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Compound cytotoxicity profiling using quantitative high-throughput screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Apoptosis, pyroptosis, and necrosis: mechanistic description of dead and dying eukaryotic cells

21 January 2018

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https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Cell death: the significance of apoptosis

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Evaluation of hepatic subcellular fractions for Alamar blue and MTT reductase activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Comparison of a novel redox dye cell growth assay to the ATP bioluminescence assay.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Comparison of alamar blue and MTT assays for high through-put screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

The mitochondrial uncoupler dicumarol disrupts the MTT assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Growth hormone-responsive DT-diaphorase-mediated bioreduction of tetrazolium salts

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Genistein inhibits tumour cell growth in vitro but enhances mitochondrial reduction of tetrazolium salts: a further pitfall in the use of the MTT assay for evaluating cell growth and survival.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Comparison of results of the CellTiter Blue, the tetrazolium (3-[4,5-dimethylthioazol-2-yl]-2,5-diphenyl tetrazolium bromide), and the lactate dehydrogenase assay applied in brain cells after exposure to advanced glycation endproducts

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Estimation of biomass in growing cell lines by adenosine triphosphate assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Development and validation of a high-throughput screen for inhibitors of SARS CoV and its application in screening of a 100,000-compound library

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Optimization procedure for small interfering RNA transfection in a 384-well format

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A simple, no-wash cell adhesion-based high-throughput assay for the discovery of small-molecule regulators of the integrin CD11b/CD18

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Development of a Ca(2+)-activated photoprotein, Photina, and its application to high-throughput screening.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Small interfering RNA and gene expression analysis using a multiplex branched DNA assay without RNA purification

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Three mechanistically distinct kinase assays compared: Measurement of intrinsic ATPase activity identified the most comprehensive set of ITK inhibitors

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

An enzyme-release assay for natural cytotoxicity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A high-throughput screen to identify inhibitors of amyloid beta-protein precursor processing.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Phage display screening of epithelial cell monolayers treated with EGTA: identification of peptide FDFWITP that modulates tight junction activity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Brucella abortus rough mutants are cytopathic for macrophages in culture

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

The metabolites of the cardioprotective drug dexrazoxane do not protect myocytes from doxorubicin-induced cytotoxicity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A very sensitive coupled luminescent assay for cytotoxicity and complement-mediated lysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

NK sensitivity of neuroblastoma cells determined by a highly sensitive coupled luminescent method

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A bioluminescent cytotoxicity assay for assessment of membrane integrity using a proteolytic biomarker

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Identification of GAPDH as a protein target of the saframycin antiproliferative agents

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Involvement of glyceraldehyde-3-phosphate dehydrogenase in tumor necrosis factor-related apoptosis-inducing ligand-mediated death of thyroid cancer cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Aurora kinases: new targets for cancer therapy

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Inhibition of KIT tyrosine kinase activity: a novel molecular approach to the treatment of KIT-positive malignancies

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Emerging role of tyrosine kinase inhibitors in the treatment of advanced renal cell cancer: a review.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Regulation of mitosis via mitotic kinases: new opportunities for cancer management

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Rhodamine-based compounds as fluorogenic substrates for serine proteinases

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

High throughput screening (HTS) for phototoxicity hazard using the in vitro 3T3 neutral red uptake assay

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Miniaturization of a Mammalian Cell-Based Assay: Luciferase Reporter Gene Readout in a 3 Microliter 1536-Well Plate

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Cytotoxicity tests for high-throughput drug discovery

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Tumor necrosis factor-alpha increases ATP content in metabolically inhibited L929 cells preceding cell death.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Oligomycin, inhibitor of the F0 part of H+-ATP-synthase, suppresses the TNF-induced apoptosis.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Evaluation of the sensitivity of three sublethal cytotoxicity assays in human HepG2 cell line using water contaminants

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A practical note on the use of cytotoxicity assays.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Different viabilities and toxicity types after 6-OHDA and Ara-C exposure evaluated by four assays in five cell lines.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Comparisons of four in vitro cytotoxicity tests: The MTT assay, NR assay, uridine incorporation and protein measurements

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

The MTT assay yields a relatively lower result of growth inhibition than the ATP assay depending on the chemotherapeutic drugs tested

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

High-throughput bioluminescence screening of ubiquitin-proteasome pathway inhibitors from chemical and natural sources

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Use of red-shifted dyes in a fluorescence polarization AKT kinase assay for detection of biological activity in natural product extracts

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Homogeneous, bioluminescent protease assays: caspase-3 as a model

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Bioluminescent assays for high-throughput screening

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A strategy for discovery of novel broad-spectrum antibacterials using a high-throughput Streptococcus pneumoniae transcription/translation screen

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Comparison of miniaturized time-resolved fluorescence resonance energy transfer and enzyme-coupled luciferase high-throughput screening assays to discover inhibitors of Rho-kinase II (ROCK-II).

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

An improved beta-lactamase reporter assay: multiplexing with a cytotoxicity readout for enhanced accuracy of hit identification

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Development of Novel Assays for Proteolytic Enzymes Using Rhodamine-Based Fluorogenic Substrates

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

A dual luciferase multiplexed high-throughput screening platform for protein-protein interactions

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Advances in high content screening for drug discovery

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

High content screening applied to large-scale cell biology

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Identification of a small molecule that induces mitotic arrest using a simplified high-content screening assay and data analysis method

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Cellular dielectric spectroscopy: a powerful new approach to label-free cellular analysis

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Dynamic and label-free monitoring of natural killer cell cytotoxic activity using electronic cell sensor arrays

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Microelectronic cell sensor assay for detection of cytotoxicity and prediction of acute toxicity

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Evaluation of cellular dielectric spectroscopy, a whole-cell, label-free technology for drug discovery on Gi-coupled GPCRs.

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

Online monitoring of cell metabolism for studying pharmacodynamic effects

21 January 2018

1 reference

https://api.crossref.org/works/10.1517%2F17460441.3.6.655

21 January 2018

Identifiers

DOI

10.1517/17460441.3.6.655

1 reference

5 December 2017

1 reference