One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment (Q50436963)

14 March 2018

title

One-step generation of triple knockout CHO cell lines using CRISPR/Cas9 and fluorescent enrichment (English)

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14 March 2018

Thomas Beuchert Kallehauge

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4

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Lasse Ebdrup Pedersen

8

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Helene Faustrup Kildegaard

9

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14 March 2018

Lise Marie Grav

Lise Marie Grav

1

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Stefan Kol

Stefan Kol

6

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Anders Holmgaard Hansen

Anders Holmgaard Hansen

5

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Gyun Min Lee

7

Gyun Min Lee

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14 March 2018

author name string

Jae Seong Lee

2

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14 March 2018

Signe Gerling

3

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publication date

10 April 2015

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14 March 2018

published in

Biotechnology Journal

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volume

10

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14 March 2018

issue

9

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14 March 2018

page(s)

1446-1456

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describes a project that uses

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Targeted modification of mammalian genomes

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

A guide to genome engineering with programmable nucleases

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Accelerating genome editing in CHO cells using CRISPR Cas9 and CRISPy, a web-based target finding tool

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Multiplex genome engineering using CRISPR/Cas systems

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

RNA-guided human genome engineering via Cas9

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

BAK and BAX deletion using zinc-finger nucleases yields apoptosis-resistant CHO cells

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

A mutant Pfu DNA polymerase designed for advanced uracil-excision DNA engineering

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Proapoptotic BAX and BAK: a requisite gateway to mitochondrial dysfunction and death

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Mitochondrial release of apoptosis-inducing factor occurs downstream of cytochrome c release in response to several proapoptotic stimuli

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human Fcgamma RIII and antibody-dependent cellular toxicity

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Methods in mammalian cell line engineering: from random mutagenesis to sequence-specific approaches.

21 January 2018

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

CHO microRNA engineering is growing up: recent successes and future challenges

21 January 2018

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

21 January 2018

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

21 January 2018

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

DNA targeting specificity of RNA-guided Cas9 nucleases

21 January 2018

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells

21 January 2018

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https://api.crossref.org/works/10.1002%2FBIOT.201500027

Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases.

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system

21 January 2018

1 reference

https://api.crossref.org/works/10.1002%2FBIOT.201500027

21 January 2018

Identifiers

DOI

10.1002/BIOT.201500027

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14 March 2018

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14 March 2018