Revealing biases inherent in recombination protocols (Q33305483)

25 July 2017

title

Revealing biases inherent in recombination protocols (English)

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25 July 2017

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6

Mark J Nelson

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25 July 2017

author name string

Javier F Chaparro-Riggers

1

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25 July 2017

Bernard Lw Loo

2

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25 July 2017

Karen M Polizzi

3

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25 July 2017

Phillip R Gibbs

4

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25 July 2017

Xiao-Song Tang

5

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25 July 2017

Andreas S Bommarius

7

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25 July 2017

publication date

14 November 2007

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25 July 2017

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25 July 2017

volume

7

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25 July 2017

page(s)

77

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A statistical analysis of random mutagenesis methods used for directed protein evolution.

25 July 2017

cites work

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Evolution of highly active enzymes by homology-independent recombination

21 June 2018

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Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains

21 June 2018

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Enantioselective biocatalysis optimized by directed evolution

21 June 2018

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Novel methods for directed evolution of enzymes: quality, not quantity

21 June 2018

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Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications

21 June 2018

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Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution

21 June 2018

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Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.

21 June 2018

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Chimeric gene library construction by a simple and highly versatile method using recombination-dependent exponential amplification.

21 June 2018

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Computational and experimental analysis of DNA shuffling

21 June 2018

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Optimized expression and specific activity of IL-12 by directed molecular evolution

21 June 2018

1 reference

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Growth factor engineering by degenerate homoduplex gene family recombination

21 June 2018

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Synthetic shuffling expands functional protein diversity by allowing amino acids to recombine independently

21 June 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

DNA family shuffling of hyperthermostable beta-glycosidases

21 June 2018

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

A monomeric red fluorescent protein

21 June 2018

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DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis

21 June 2018

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Analysis of shuffled gene libraries

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

GFP-like chromoproteins as a source of far-red fluorescent proteins

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Investigating and Engineering Enzymes by Genetic Selection

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Improved biocatalysts by directed evolution and rational protein design

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Predicting crossover generation in DNA shuffling.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Breeding of retroviruses by DNA shuffling for improved stability and processing yields

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Molecular breeding of viruses

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Molecular breeding of carotenoid biosynthetic pathways

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

A combinatorial approach to hybrid enzymes independent of DNA homology

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Incremental truncation as a strategy in the engineering of novel biocatalysts

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

DNA shuffling of subgenomic sequences of subtilisin

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Evolution of a cytokine using DNA family shuffling

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Combinatorial protein engineering by incremental truncation

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Molecular evolution by staggered extension process (StEP) in vitro recombination

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

DNA shuffling of a family of genes from diverse species accelerates directed evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Functional and nonfunctional mutations distinguished by random recombination of homologous genes

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Optimization of DNA shuffling for high fidelity recombination

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Amino acid sequence determinants of beta-lactamase structure and activity

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Rapid evolution of a protein in vitro by DNA shuffling

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Template-switching during DNA synthesis byThermus aquaticusDNA polymerase I

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

DNA recombination during PCR.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Factors affecting PCR-mediated recombination.

21 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Improved green fluorescent protein by molecular evolution using DNA shuffling

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Strategies for the in vitro evolution of protein function: enzyme evolution by random recombination of improved sequences

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Molecular evolution of an arsenate detoxification pathway by DNA shuffling.

31 October 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2203992

Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling

31 October 2018

1 reference

12 December 2020

DNA shuffling method for generating estrogen receptor alpha and beta chimeras in yeast

1 reference

12 December 2020

Novel family shuffling methods for the in vitro evolution of enzymes

1 reference

12 December 2020

Creating seamless junctions independent of restriction sites in PCR cloning

1 reference

12 December 2020

Identifiers

DOI

10.1186/1472-6750-7-77

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Dimensions Publication ID

25 July 2017

0 references

1 reference

25 July 2017

1 reference