Rigidification of the autolysis loop enhances Na+ binding to thrombin (Q27667649)

2

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Zhiwei Chen

3

0 references

Alaji Bah

4

0 references

Enrico Di Cera

Structural basis of the cofactor- and substrate-assisted activation of human coagulation factor IXa

5

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publication date

November 2011

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published in

Biophysical Chemistry

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volume

159

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issue

1

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page(s)

6-13

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cites work

1 reference

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Mutant N143P Reveals How Na+ Activates Thrombin

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Mechanism of the Anticoagulant Activity of Thrombin Mutant W215A/E217A

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Stabilization of the E* Form Turns Thrombin into an Anticoagulant

20 March 2017

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Na+ binding to meizothrombin desF1

20 March 2017

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Structural identification of the pathway of long-range communication in an allosteric enzyme

20 March 2017

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Important role of the cys-191 cys-220 disulfide bond in thrombin function and allostery

20 March 2017

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Crystal structures of murine thrombin in complex with the extracellular fragments of murine protease-activated receptors PAR3 and PAR4

20 March 2017

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Structural basis of Na+ activation mimicry in murine thrombin

20 March 2017

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Crystal structure of the anticoagulant slow form of thrombin

20 March 2017

1 reference

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Thermodynamic linkage between the S1 site, the Na+ site, and the Ca2+ site in the protease domain of human activated protein C (APC). Sodium ion in the APC crystal structure is coordinated to four carbonyl groups from two separate loops

20 March 2017

1 reference

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The molecular environment of the Na+ binding site of thrombin

20 March 2017

1 reference

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How potassium affects the activity of the molecular chaperone Hsc70. II. Potassium binds specifically in the ATPase active site

20 March 2017

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Coot: model-building tools for molecular graphics

20 March 2017

1 reference

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Refinement of macromolecular structures by the maximum-likelihood method

20 March 2017

1 reference

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The CCP4 suite: programs for protein crystallography

20 March 2017

1 reference

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Stereochemical quality of protein structure coordinates

20 March 2017

1 reference

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The dynamics of thrombin formation

20 March 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Thrombin signalling and protease-activated receptors

20 March 2017

1 reference

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Redesigning allosteric activation in an enzyme

7 April 2017

1 reference

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Thrombin as an anticoagulant

29 September 2017

1 reference

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Evidence of the E*−E Equilibrium from Rapid Kinetics of Na+ Binding to Activated Protein C and Factor Xa*

29 September 2017

1 reference

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29 September 2017

1 reference

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Thrombin as procoagulant and anticoagulant

29 September 2017

1 reference

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Expression of allosteric linkage between the sodium ion binding site and exosite I of thrombin during prothrombin activation

29 September 2017

1 reference

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Relative antithrombotic and antihemostatic effects of protein C activator versus low-molecular-weight heparin in primates.

29 September 2017

1 reference

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The status of new anticoagulants

29 September 2017

1 reference

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An overview of the structure and function of thrombin

29 September 2017

1 reference

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Structure and interaction modes of thrombin

29 September 2017

1 reference

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A structural perspective on enzymes activated by monovalent cations

29 September 2017

1 reference

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Protease-activated receptors in hemostasis, thrombosis and vascular biology

29 September 2017

1 reference

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Na+ site in blood coagulation factor IXa: effect on catalysis and factor VIIIa binding

29 September 2017

1 reference

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The critical role of the 185-189-loop in the factor Xa interaction with Na+ and factor Va in the prothrombinase complex.

29 September 2017

1 reference

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Redesigning the monovalent cation specificity of an enzyme

29 September 2017

1 reference

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29 September 2017

1 reference

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Prothrombinase assembly and S1 site occupation restore the catalytic activity of FXa impaired by mutation at the sodium-binding site

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Sodium binding site of factor Xa: role of sodium in the prothrombinase complex

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Residue 225 determines the Na(+)-induced allosteric regulation of catalytic activity in serine proteases

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

The use of fluorescence methods to monitor unfolding transitions in proteins

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

An allosteric switch controls the procoagulant and anticoagulant activities of thrombin

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Engineered thrombin aims to take on heparin

29 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Mechanism of Na(+) binding to thrombin resolved by ultra-rapid kinetics.

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Effect of Na+ binding on the conformation, stability and molecular recognition properties of thrombin

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Probing the conformational state of apomyoglobin by limited proteolysis.

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Evidence for common structural changes in thrombin induced by active-site or exosite binding

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Conversion of thrombin into an anticoagulant by protein engineering

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Effect of monovalent cations on the pre-steady-state kinetic parameters of the plasma protease bovine activated protein C.

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Kinetic mechanism for stimulation by monovalent cations of the amidase activity of the plasma protease bovine activated protein C.

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Thrombin is a Na(+)-activated enzyme

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Prothrombin Salakta: substitution of glutamic acid-466 by alanine reduces the fibrinogen clotting activity and the esterase activity

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

The effect of metal ions on the amidolytic activity of human factor Xa (Activated Stuart-Prower Factor)

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Prothrombin Frankfurt: a dysfunctional prothrombin characterized by substitution of Glu-466 by Ala.

2 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Thrombin mutant W215A/E217A acts as a platelet GPIb antagonist.

19 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

High resolution structures of p-aminobenzamidine- and benzamidine-VIIa/soluble tissue factor: unpredicted conformation of the 192-193 peptide bond and mapping of Ca2+, Mg2+, Na+, and Zn2+ sites in factor VIIa

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Murine thrombin lacks Na+ activation but retains high catalytic activity.

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Limited generation of activated protein C during infusion of the protein C activator thrombin analog W215A/E217A in primates.

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Exosite-interactive regions in the A1 and A2 domains of factor VIII facilitate thrombin-catalyzed cleavage of heavy chain

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Molecular dissection of Na+ binding to thrombin.

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Residue Asp-189 controls both substrate binding and the monovalent cation specificity of thrombin

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Thrombin activation of factor XI on activated platelets requires the interaction of factor XI and platelet glycoprotein Ib alpha with thrombin anion-binding exosites I and II, respectively

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Dissecting substrate recognition by thrombin using the inactive mutant S195A.

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Prothrombin Scranton: substitution of an amino acid residue involved in the binding of Na+ (LYS-556 to THR) leads to dysprothrombinemia.

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Thermodynamics of Na+ binding to coagulation serine proteases

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

An extensive interaction interface between thrombin and factor V is required for factor V activation

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Protein engineering thrombin for optimal specificity and potency of anticoagulant activity in vivo

27 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=3150630

Identification of a binding site for quaternary amines in factor Xa

27 November 2018

1 reference

12 December 2020

Thermodynamic linkage between the S1 site, the Na+ site, and the Ca2+ site in the protease domain of human coagulation factor xa. Studies on catalytic efficiency and inhibitor binding

1 reference

12 December 2020

Rational design of a potent anticoagulant thrombin

1 reference

12 December 2020

Role of residue Phe225 in the cofactor-mediated, allosteric regulation of the serine protease coagulation factor VIIa

1 reference

12 December 2020

Molecular mapping of thrombin-receptor interactions

1 reference

12 December 2020

The thrombin mutant W215A/E217A shows safe and potent anticoagulant and antithrombotic effects in vivo

1 reference

12 December 2020

Quantification of cation-pi interactions in protein-ligand complexes: crystal-structure analysis of Factor Xa bound to a quaternary ammonium ion ligand

1 reference

12 December 2020

Prothrombin Saint-Denis: a natural variant with a point mutation resulting in Asp to Glu substitution at position 552 in prothrombin

1 reference

12 December 2020

Prothrombin deficiency caused by compound heterozygosity for two novel mutations in the prothrombin gene associated with a bleeding tendency

1 reference

12 December 2020

The influence of sodium ion binding on factor IXa activity

1 reference

12 December 2020

Crystal structure of thrombin in a self-inhibited conformation

1 reference

12 December 2020

Rapid kinetics of Na+ binding to thrombin

1 reference

12 December 2020

Role of the alpha-helix 163-170 in factor Xa catalytic activity

1 reference

12 December 2020

Processing of X-ray diffraction data collected in oscillation mode

1 reference

12 December 2020

Enzymes activated by monovalent cations

1 reference

12 December 2020

Stimulation of the amidase and esterase activity of activated bovine plasma protein C by monovalent cations

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12 December 2020

Kinetic studies of the role of monovalent cations in the amidolytic activity of activated bovine plasma protein C

1 reference

12 December 2020

The Na+ Binding Site of Thrombin

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12 December 2020

Release of fibrinopeptides by the slow and fast forms of thrombin

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12 December 2020

Large heat capacity change in a protein-monovalent cation interaction

1 reference

12 December 2020

Rational engineering of activity and specificity in a serine protease

1 reference

12 December 2020

Selective loss of fibrinogen clotting in a loop-less thrombin

1 reference

12 December 2020

Kinetic pathway for the slow to fast transition of thrombin. Evidence of linked ligand binding at structurally distinct domains

1 reference

12 December 2020

Prothrombin Greenville, Arg517-->Gln, identified in an individual heterozygous for dysprothrombinemia

1 reference

12 December 2020

Identification and characterization of the sodium-binding site of activated protein C

1 reference

12 December 2020

10.1016/J.BPC.2011.04.003

Identifiers

DOI

1 reference

1 reference

1 reference

PubMed publication ID

21536369

1 reference