Recent developments in the use of differential scanning fluorometry in protein and small molecule discovery and characterization (Q26996528)

language of work or name

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September 2013

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Expert Opinion on Drug Discovery

published in

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volume

8

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issue

9

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page(s)

1071-82

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Computational identification of a transiently open L1/S3 pocket for reactivation of mutant p53

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21 March 2017

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High-throughput thermal scanning: a general, rapid dye-binding thermal shift screen for protein engineering

21 March 2017

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Structural and biochemical characterization of an atypical short-chain dehydrogenase/reductase reveals an unusual cofactor preference

21 March 2017

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Ternary complex structures of human farnesyl pyrophosphate synthase bound with a novel inhibitor and secondary ligands provide insights into the molecular details of the enzyme’s active site closure

21 March 2017

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Family-wide chemical profiling and structural analysis of PARP and tankyrase inhibitors

21 March 2017

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A Molecular Mechanism for Bacterial Susceptibility to Zinc

21 March 2017

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Calcium modulates force sensing by the von Willebrand factor A2 domain

21 March 2017

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Structure and Mechanism of Human UDP-glucose 6-Dehydrogenase

21 March 2017

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Molecular basis for the high-affinity binding and stabilization of firefly luciferase by PTC124

21 March 2017

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Differential scanning fluorometry signatures as indicators of enzyme inhibitor mode of action: case study of glutathione S-transferase

21 March 2017

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The use of differential scanning fluorimetry to detect ligand interactions that promote protein stability

21 March 2017

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High-density miniaturized thermal shift assays as a general strategy for drug discovery

7 April 2017

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Thermodynamics of binding interactions in the rational drug design process

7 April 2017

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Bromo-deaza-SAH: a potent and selective DOT1L inhibitor

28 September 2017

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Pharmacological chaperones as therapeutics for lysosomal storage diseases

28 September 2017

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Resolution of the interaction mechanisms and characteristics of non-nucleoside inhibitors of hepatitis C virus polymerase

28 September 2017

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Protein quality control acts on folding intermediates to shape the effects of mutations on organismal fitness

28 September 2017

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High-throughput melting-temperature analysis of a monoclonal antibody by differential scanning fluorimetry in the presence of surfactants

28 September 2017

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The stability of 2-state, 3-state and more-state proteins from simple spectroscopic techniques... plus the structure of the equilibrium intermediates at the same time.

28 September 2017

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Evaluating Drosophila p53 as a model system for studying cancer mutations

28 September 2017

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Functional Rescue of F508del-CFTR Using Small Molecule Correctors

28 September 2017

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Ligand-receptor interaction platforms and their applications for drug discovery.

28 September 2017

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Affinity-based screening techniques: their impact and benefit to increase the number of high quality leads

28 September 2017

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Discovery, structure-activity relationship, and biological evaluation of noninhibitory small molecule chaperones of glucocerebrosidase

28 September 2017

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Identification of HIV-1 inhibitors targeting the nucleocapsid protein.

28 September 2017

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A FRET-based screening assay for nucleic acid ligands.

28 September 2017

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Thermodynamic studies for drug design and screening

28 September 2017

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Thermal denaturation assays in chemical biology

28 September 2017

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High-throughput measurement, correlation analysis, and machine-learning predictions for pH and thermal stabilities of Pfizer-generated antibodies

28 September 2017

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Quantitation of protein-protein interactions by thermal stability shift analysis

28 September 2017

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Identification of a NBD1-binding pharmacological chaperone that corrects the trafficking defect of F508del-CFTR.

28 September 2017

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Evaluation of quinazoline analogues as glucocerebrosidase inhibitors with chaperone activity

28 September 2017

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Higher throughput calorimetry: opportunities, approaches and challenges

28 September 2017

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Fluorescence-based thermal shift assays

28 September 2017

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Biophysical characterization of recombinant proteins: a key to higher structural genomics success

28 September 2017

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Use of thermal melt curves to assess the quality of enzyme preparations

28 September 2017

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Identification and characterization of ambroxol as an enzyme enhancement agent for Gaucher disease

28 September 2017

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Buffer optimization of thermal melt assays of Plasmodium proteins for detection of small-molecule ligands

28 September 2017

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High throughput methods of assessing protein stability and aggregation

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

Mechanism of PTC124 activity in cell-based luciferase assays of nonsense codon suppression

28 September 2017

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A specific mechanism for nonspecific activation in reporter-gene assays

28 September 2017

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Identification of trinucleotide repeat ligands with a FRET melting assay

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

ATLAS--a high-throughput affinity-based screening technology for soluble proteins: technology application using p38 MAP kinase

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination

28 September 2017

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Measurements of binding thermodynamics in drug discovery

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

Thermodynamic stability of carbonic anhydrase: measurements of binding affinity and stoichiometry using ThermoFluor.

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

1,4-Benzodiazepine-2,5-diones as small molecule antagonists of the HDM2-p53 interaction: discovery and SAR.

28 September 2017

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Evaluation of fluorescence-based thermal shift assays for hit identification in drug discovery

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

Applications of calorimetric methods to drug discovery and the study of protein interactions

28 September 2017

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Fluorescence resonance energy transfer as a probe for G-quartet formation by a telomeric repeat

28 September 2017

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Study of strong to ultratight protein interactions using differential scanning calorimetry

28 September 2017

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Small-molecule inhibition of MLL activity by disruption of its interaction with WDR5

28 September 2017

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https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4906806

Correction of both NBD1 energetics and domain interface is required to restore ΔF508 CFTR folding and function

31 May 2018

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Measurement of nanomolar dissociation constants by titration calorimetry and thermal shift assay - radicicol binding to Hsp90 and ethoxzolamide binding to CAII

31 May 2018

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Leveraging the contribution of thermodynamics in drug discovery with the help of fluorescence-based thermal shift assays.

31 May 2018

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https://api.crossref.org/works/10.1517%2F17460441.2013.806479

Application of high-throughput isothermal denaturation to assess protein stability and screen for ligands

21 January 2018

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https://api.crossref.org/works/10.1517%2F17460441.2013.806479

Identification of ligand binding by protein stabilization: comparison of ATLAS with biophysical and enzymatic methods

21 January 2018

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Real-time protein unfolding: a method for determining the kinetics of native protein denaturation using a quantitative real-time thermocycler

21 January 2018

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High throughput formulation screening for global aggregation behaviors of three monoclonal antibodies

21 January 2018

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https://api.crossref.org/works/10.1517%2F17460441.2013.806479

High throughput thermostability screening of monoclonal antibody formulations

21 January 2018

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https://api.crossref.org/works/10.1517%2F17460441.2013.806479

Comparison of fluorescence and light scattering based methods to assess formation and stability of protein–protein complexes

21 January 2018

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https://api.crossref.org/works/10.1517%2F17460441.2013.806479

Screening and characterization of human monoglyceride lipase active site inhibitors using orthogonal binding and functional assays

21 January 2018

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Assessing the differential affinity of small molecules for noncanonical DNA structures

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12 December 2020

Formulation Development of Therapeutic Monoclonal Antibodies Using High-Throughput Fluorescence and Static Light Scattering Techniques: Role of Conformational and Colloidal Stability

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Heat does not come in different colours: entropy-enthalpy compensation, free energy windows, quantum confinement, pressure perturbation calorimetry, solvation and the multiple causes of heat capacity effects in biomolecular interactions

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